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In Vitro Mutagenesis

Overview of attention for book
Cover of 'In Vitro Mutagenesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.
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    Chapter 2 Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9.
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    Chapter 3 Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.
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    Chapter 4 All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.
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    Chapter 5 CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium.
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    Chapter 6 Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.
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    Chapter 7 Generation of Stable Knockout Mammalian Cells by TALEN-Mediated Locus-Specific Gene Editing.
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    Chapter 8 Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned Technology.
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    Chapter 9 Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes.
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    Chapter 10 Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells.
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    Chapter 11 Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.
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    Chapter 12 In Silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships.
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    Chapter 13 In Silico Prediction of Deleteriousness for Nonsynonymous and Splice-Altering Single Nucleotide Variants in the Human Genome.
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    Chapter 14 In Silico Methods for Analyzing Mutagenesis Targets.
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    Chapter 15 Methods for Detecting Critical Residues in Proteins.
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    Chapter 16 A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants.
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    Chapter 17 Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
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    Chapter 18 Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors.
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    Chapter 19 PERMutation Using Transposase Engineering (PERMUTE): A Simple Approach for Constructing Circularly Permuted Protein Libraries.
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    Chapter 20 Transposon Insertion Mutagenesis for Archaeal Gene Discovery.
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    Chapter 21 Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis.
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    Chapter 22 Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.
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    Chapter 23 Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis.
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    Chapter 24 Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
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    Chapter 25 Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
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    Chapter 26 An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology.
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    Chapter 27 Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function.
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    Chapter 28 Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL.
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    Chapter 29 Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein-Cofactor and Protein-Protein Functional Relationships.
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    Chapter 30 Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions.
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    Chapter 31 Use of Random and Site-Directed Mutagenesis to Probe Protein Structure-Function Relationships: Applied Techniques in the Study of Helicobacter pylori.
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    Chapter 32 Novel Random Mutagenesis Method for Directed Evolution.
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    Chapter 33 Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.
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    Chapter 34 Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR).
Attention for Chapter 6: Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

blogs
1 blog
wikipedia
1 Wikipedia page

Citations

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6 Dimensions

Readers on

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50 Mendeley
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Chapter title
Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.
Chapter number 6
Book title
In Vitro Mutagenesis
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6472-7_6
Pubmed ID
27709570
Book ISBNs
978-1-4939-6470-3, 978-1-4939-6472-7
Authors

Shen, Bang, Brown, Kevin, Long, Shaojun, Sibley, L David, Bang Shen, Kevin Brown, Shaojun Long, L. David Sibley

Editors

Andrew Reeves

Abstract

Efficient and site-specific alteration of the genome is key to decoding and altering the genomic information of an organism. Over the last couple of years, the RNA-guided Cas9 nucleases derived from the prokaryotic type 2 CRISPR (clustered regularly interspaced short palindromic repeats) systems have drastically improved our ability to engineer the genomes of a variety of organisms including Toxoplasma gondii. In this chapter, we describe detailed protocols for using the CRISPR/Cas9 system adapted from Streptococcus pyogenes to perform efficient genetic manipulations in T. gondii such as gene disruption, gene tagging and genetic complementation. The technical details of the strategy, including CRISPR plasmid construction, target construct generation, parasite transfection and positive clone identification are also provided. These methods are easy to customize to any gene of interest (GOI) and will greatly accelerate studies on this important pathogen.

View on publisher site
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 36%
Student > Master 8 16%
Researcher 4 8%
Professor 2 4%
Student > Doctoral Student 2 4%
Other 7 14%
Unknown 9 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 34%
Agricultural and Biological Sciences 17 34%
Immunology and Microbiology 4 8%
Engineering 2 4%
Social Sciences 1 2%
Other 1 2%
Unknown 8 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2016.
All research outputs
#3,800,207
of 22,890,496 outputs
Outputs from Methods in molecular biology
#973
of 13,134 outputs
Outputs of similar age
#76,274
of 420,414 outputs
Outputs of similar age from Methods in molecular biology
#112
of 1,074 outputs
Altmetric has tracked 22,890,496 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,134 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,414 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.

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