| Title |
Chromosome Architecture
|
|---|---|
| Published by |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3631-1 |
| Pubmed ID | |
| ISBNs |
978-1-4939-3629-8, 978-1-4939-3631-1
|
| Authors |
Springall, Luke, Inchingolo, Alessio V, Kad, Neil M |
| Editors |
Mark C. Leake |
| Abstract |
Many protein interactions with DNA require specific sequences; however, how these sequences are located remains uncertain. DNA normally appears bundled in solution but, to study DNA-protein interactions, the DNA needs to be elongated. Using fluidics single DNA strands can be efficiently and rapidly elongated between beads immobilized on a microscope slide surface. Such "DNA tightropes" offer a valuable method to study protein search mechanisms. Real-time fluorescence imaging of these interactions provides quantitative descriptions of search mechanism at the single molecule level. In our lab, we use this method to study the complex process of nucleotide excision DNA repair to determine mechanisms of damage detection, lesion removal, and DNA excision. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 24% |
| Canada | 1 | 6% |
| Unknown | 12 | 71% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 65% |
| Scientists | 6 | 35% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 9% |
| Student > Bachelor | 3 | 6% |
| Lecturer | 1 | 2% |
| Student > Master | 1 | 2% |
| Unknown | 38 | 81% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Agricultural and Biological Sciences | 4 | 9% |
| Neuroscience | 1 | 2% |
| Unknown | 38 | 81% |