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Mendeley readers
Attention Score in Context
| Title |
Sulforhodamine Nanothermometer for Multiparametric Fluorescence Lifetime Imaging Microscopy
|
|---|---|
| Published in |
Analytical Chemistry, October 2016
|
| DOI | 10.1021/acs.analchem.6b02675 |
| Pubmed ID | |
| Authors |
James Jenkins, Sergey M. Borisov, Dmitri B. Papkovsky, Ruslan I. Dmitriev |
| Abstract |
Live cells function within narrow limits of physiological temperature (T), O2 and metabolite concentrations. We have designed a cell-permeable T-sensitive fluorescence lifetime-based nanoprobe based on lipophilic sulforhodamine, which stains 2D and 3D cell models, shows cytoplasmic localization and robust response to T (~0.037 ns/K). Subsequently, we evaluated the probe and FLIM technique for combined imaging of T and O2 gradients in metabolically active cells. We found that in adherent 2D culture of HCT116 cells intracellular T and O2 are close to ambient values. However in 3D spheroid structures having size >200 μm, T and O2 gradients become pronounced. These microgradients can be enhanced by treatment with mitochondrial uncouplers or dissipated by drug-induced disaggregation of the spheroids. Thus, we demonstrate the existence of local microgradients of T in 3D cell models and utility of combined imaging of O2 and T. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 67% |
| Belgium | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 21% |
| Researcher | 11 | 21% |
| Student > Master | 8 | 15% |
| Student > Postgraduate | 3 | 6% |
| Professor > Associate Professor | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 19 | 37% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Physics and Astronomy | 4 | 8% |
| Medicine and Dentistry | 2 | 4% |
| Economics, Econometrics and Finance | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 17 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 March 2023.
All research outputs
#13,497,650
of 23,477,147 outputs
Outputs from Analytical Chemistry
#17,862
of 26,783 outputs
Outputs of similar age
#165,145
of 321,973 outputs
Outputs of similar age from Analytical Chemistry
#108
of 245 outputs
Altmetric has tracked 23,477,147 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 26,783 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 321,973 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 245 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.