| Title |
Tenogenic differentiation of equine adipose-tissue-derived stem cells under the influence of tensile strain, growth differentiation factors and various oxygen tensions
|
|---|---|
| Published in |
Cell and Tissue Research, February 2013
|
| DOI | 10.1007/s00441-013-1574-1 |
| Pubmed ID | |
| Authors |
Oksana Raabe, K. Shell, D. Fietz, C. Freitag, A. Ohrndorf, H. J. Christ, S. Wenisch, S. Arnhold |
| Abstract |
Mesenchymal stem cells have become extremely interesting for regenerative medicine and tissue engineering in the horse. Stem cell therapy has been proven to be a powerful and successful instrument, in particular for the healing of tendon lesions. We pre-differentiated equine adipose-tissue-derived stem cells (ASCs) in a collagen I gel scaffold by applying tensile strain, growth differentiation factors (GDFs) and various oxygen tensions in order to determine the optimal conditions for in vitro differentiation toward the tenogenic lineage. We compared the influence of 3% versus 21% oxygen tension, the use of GDF 5, GDF 6 and GDF 7 and the application of uniaxial tensile strain versus no mechanical stimulation on differentiation results as evaluated by cell morphology and by the expression of the tendon-relevant genes collagen I, collagen III, cartilage oligomeric matrix protein and scleraxis. The best results were obtained with an oxygen tension of 21%, tensile stimulation and supplementation with GDF 5 or GDF 7. This approach raises the hope that the in vivo application of pre-differentiated stem cells will improve healing and recovery time in comparison with treatment involving undifferentiated stem cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 2% |
| Italy | 1 | 1% |
| Brazil | 1 | 1% |
| Iran, Islamic Republic of | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 76 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 24% |
| Student > Master | 16 | 20% |
| Researcher | 13 | 16% |
| Student > Bachelor | 4 | 5% |
| Other | 4 | 5% |
| Other | 13 | 16% |
| Unknown | 12 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 27% |
| Medicine and Dentistry | 11 | 13% |
| Engineering | 10 | 12% |
| Veterinary Science and Veterinary Medicine | 6 | 7% |
| Biochemistry, Genetics and Molecular Biology | 5 | 6% |
| Other | 10 | 12% |
| Unknown | 18 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 February 2013.
All research outputs
#18,563,902
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,645
of 2,279 outputs
Outputs of similar age
#143,247
of 195,145 outputs
Outputs of similar age from Cell and Tissue Research
#11
of 36 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 25th percentile – i.e., 25% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 195,145 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.