| Title |
Genome-wide enrichment screening reveals multiple targets and resistance genes for triclosan in Escherichia coli
|
|---|---|
| Published in |
Journal of Microbiology, November 2012
|
| DOI | 10.1007/s12275-012-2439-0 |
| Pubmed ID | |
| Authors |
Byung Jo Yu, Jung Ae Kim, Hyun Mok Ju, Soo-Kyung Choi, Seung Jin Hwang, Sungyoo Park, EuiJoong Kim, Jae-Gu Pan |
| Abstract |
Triclosan is a widely used biocide effective against different microorganisms. At bactericidal concentrations, triclosan appears to affect multiple targets, while at bacteriostatic concentrations, triclosan targets FabI. The site-specific antibiotic-like mode-of-action and a widespread use of triclosan in household products claimed to possibly induce cross-resistance to other antibiotics. Thus, we set out to define more systematically the genes conferring resistance to triclosan; A genomic library of Escherichia coli strain W3110 was constructed and enriched in a selective medium containing a lethal concentration of triclosan. The genes enabling growth in the presence of triclosan were identified by using a DNA microarray and confirmed consequently by ASKA clones overexpressing the selected 62 candidate genes. Among these, forty-seven genes were further confirmed to enhance the resistance to triclosan; these genes, including the FabI target, were involved in inner or outer membrane synthesis, cell-surface material synthesis, transcriptional activation, sugar phosphotransferase (PTS) systems, various transporter systems, cell division, and ATPase and reductase/dehydrogenase reactions. In particular, overexpression of pgsA, rcsA, or gapC conferred to E. coli cells a similar level of triclosan resistance induced by fabI overexpression. These results indicate that triclosan may have multiple targets other than well-known FabI and that there are several undefined novel mechanisms for the resistance development to triclosan, thus probably inducing cross antibiotic resistance. |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Indonesia | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 49 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 27% |
| Student > Ph. D. Student | 10 | 20% |
| Student > Bachelor | 8 | 16% |
| Student > Doctoral Student | 2 | 4% |
| Other | 2 | 4% |
| Other | 8 | 16% |
| Unknown | 7 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 35% |
| Biochemistry, Genetics and Molecular Biology | 11 | 22% |
| Engineering | 4 | 8% |
| Immunology and Microbiology | 4 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 8 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 February 2013.
All research outputs
#5,004,272
of 23,975,976 outputs
Outputs from Journal of Microbiology
#86
of 842 outputs
Outputs of similar age
#36,710
of 186,969 outputs
Outputs of similar age from Journal of Microbiology
#2
of 8 outputs
Altmetric has tracked 23,975,976 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 842 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 89% of its peers.
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