| Title |
Expression of Two Novel β-Glucosidases from Chaetomium atrobrunneum in Trichoderma reesei and Characterization of the Heterologous Protein Products
|
|---|---|
| Published in |
Molecular Biotechnology, October 2016
|
| DOI | 10.1007/s12033-016-9981-7 |
| Pubmed ID | |
| Authors |
Ana C. Colabardini, Mari Valkonen, Anne Huuskonen, Matti Siika-aho, Anu Koivula, Gustavo H. Goldman, Markku Saloheimo |
| Abstract |
Two novel GH3 family thermostable β-glucosidases from the filamentous fungus Chaetomium atrobrunneum (CEL3a and CEL3b) were expressed in Trichoderma reesei, purified by two-step ion exchange chromatography, and characterized. Both enzymes were active over a wide range of pH as compared to Neurospora crassa β-glucosidase GH3-3, which was also expressed in T. reesei and purified. The optimum temperature of both C. atrobrunneum enzymes was around 60 °C at pH 5, and both enzymes had better thermal and pH stability and higher resistance to metallic compounds and to glucose inhibition than GH3-3. They also showed higher activity against oligosaccharides composed of glucose units and linked with β-1,4-glycosidic bonds and moreover, had higher affinity for cellotriose over cellobiose. In hydrolysis tests against Avicel cellulose and steam-exploded sugarcane bagasse, performed at 45 °C, particularly the CEL3a enzyme performed similarly to N. crassa GH3-3 β-glucosidase. Taking into account the thermal stability of the C. atrobrunneum β-glucosidases, they both represent promising alternatives as enzyme mixture components for improved cellulose saccharification at elevated temperatures. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 20% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Doctoral Student | 5 | 12% |
| Student > Bachelor | 3 | 7% |
| Other | 2 | 5% |
| Other | 7 | 17% |
| Unknown | 10 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 24% |
| Biochemistry, Genetics and Molecular Biology | 10 | 24% |
| Medicine and Dentistry | 2 | 5% |
| Materials Science | 1 | 2% |
| Unspecified | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 17 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2022.
All research outputs
#4,593,046
of 22,757,090 outputs
Outputs from Molecular Biotechnology
#113
of 958 outputs
Outputs of similar age
#77,548
of 319,602 outputs
Outputs of similar age from Molecular Biotechnology
#4
of 10 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 958 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 87% of its peers.
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We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.