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X Demographics
Mendeley readers
Attention Score in Context
| Title |
The effect of forced growth of cells into 3D spheres using low attachment surfaces on the acquisition of stemness properties
|
|---|---|
| Published in |
Clinical Materials, February 2013
|
| DOI | 10.1016/j.biomaterials.2013.01.044 |
| Pubmed ID | |
| Authors |
Guannan Su, Yannan Zhao, Jianshu Wei, Jin Han, Lei Chen, Zhifeng Xiao, Bing Chen, Jianwu Dai |
| Abstract |
Embryonic stem cells (ESCs) and neural progenitor cells form three-dimensional (3D) colonies or spheres in vitro, and 3D sphere is reported to help maintaining the stemness of stem cells, but the effect of 3D sphere formation on cell reprogramming remains unknown. Here we examined whether 3D sphere culture have any impact on the differentiated cells. We cultured bladder cancer cell RT4 and non-cancerous cell HEK293 on the low attachment dishes coated with soft agarose. When grown on this low attachment dish, cells spontaneously aggregated to form 3D spheres. Data showed that 3D sphere formation promoted the expression of reprogramming factors. Sphere formation of RT4 cells induced cancer stem cell characteristics including higher SP cell percentage, higher metastasis ability and higher tumorigenicity. HEK293 spheres showed upregulation of kidney progenitor cell markers and partially acquired characteristics of ESCs including upregulation of alkaline phosphatase activity, ES cell markers, three germ layer markers and tumorigenicity. The findings suggested that forced growth into 3D spheres by the low attachment surface could induce cells to acquire stemness properties. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 1% |
| France | 1 | 1% |
| Germany | 1 | 1% |
| Unknown | 88 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 27% |
| Student > Master | 13 | 14% |
| Student > Bachelor | 13 | 14% |
| Researcher | 9 | 10% |
| Professor > Associate Professor | 7 | 8% |
| Other | 14 | 15% |
| Unknown | 10 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 27% |
| Biochemistry, Genetics and Molecular Biology | 19 | 21% |
| Engineering | 10 | 11% |
| Medicine and Dentistry | 8 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Other | 7 | 8% |
| Unknown | 19 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2023.
All research outputs
#3,561,046
of 25,373,627 outputs
Outputs from Clinical Materials
#1,053
of 10,751 outputs
Outputs of similar age
#35,668
of 292,398 outputs
Outputs of similar age from Clinical Materials
#12
of 115 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,751 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 292,398 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 115 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.