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A New Insect-Specific Flavivirus from Northern Australia Suppresses Replication of West Nile Virus and Murray Valley Encephalitis Virus in Co-infected Mosquito Cells

Overview of attention for article published in PLOS ONE, February 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

Mentioned by

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13 X users
facebook
1 Facebook page
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2 Wikipedia pages
googleplus
1 Google+ user

Citations

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187 Dimensions

Readers on

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199 Mendeley
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Title
A New Insect-Specific Flavivirus from Northern Australia Suppresses Replication of West Nile Virus and Murray Valley Encephalitis Virus in Co-infected Mosquito Cells
Published in
PLOS ONE, February 2013
DOI 10.1371/journal.pone.0056534
Pubmed ID
Authors

Jody Hobson-Peters, Alice Wei Yee Yam, Jennifer Wei Fei Lu, Yin Xiang Setoh, Fiona J. May, Nina Kurucz, Susan Walsh, Natalie A. Prow, Steven S. Davis, Richard Weir, Lorna Melville, Neville Hunt, Richard I. Webb, Bradley J. Blitvich, Peter Whelan, Roy A. Hall

Abstract

Recent reports of a novel group of flaviviruses that replicate only in mosquitoes and appear to spread through insect populations via vertical transmission have emerged from around the globe. To date, there is no information on the presence or prevalence of these insect-specific flaviviruses (ISFs) in Australian mosquito species. To assess whether such viruses occur locally, we used reverse transcription-polymerase chain reaction (RT-PCR) and flavivirus universal primers that are specific to the NS5 gene to detect these viruses in mosquito pools collected from the Northern Territory. Of 94 pools of mosquitoes, 13 were RT-PCR positive, and of these, 6 flavivirus isolates were obtained by inoculation of mosquito cell culture. Sequence analysis of the NS5 gene revealed that these isolates are genetically and phylogenetically similar to ISFs reported from other parts of the world. The entire coding region of one isolate (designated 56) was sequenced and shown to have approximately 63.7% nucleotide identity and 66.6% amino acid identity with its closest known relative (Nakiwogo virus) indicating that the prototype Australian ISF represents a new species. All isolates were obtained from Coquillettidia xanthogaster mosquitoes. The new virus is tentatively named Palm Creek virus (PCV) after its place of isolation. We also demonstrated that prior infection of cultured mosquito cells with PCV suppressed subsequent replication of the medically significant West Nile and Murray Valley encephalitis viruses by 10-43 fold (1 to 1.63 log) at 48 hr post-infection, suggesting that superinfection exclusion can occur between ISFs and vertebrate-infecting flaviviruses despite their high level of genetic diversity. We also generated several monoclonal antibodies (mAbs) that are specific to the NS1 protein of PCV, and these represent the first ISF-specific mAbs reported to date.

X Demographics

X Demographics

The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 199 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 2%
Portugal 1 <1%
France 1 <1%
Unknown 193 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 36 18%
Student > Master 30 15%
Student > Ph. D. Student 29 15%
Student > Bachelor 20 10%
Student > Doctoral Student 9 5%
Other 26 13%
Unknown 49 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 49 25%
Biochemistry, Genetics and Molecular Biology 36 18%
Immunology and Microbiology 23 12%
Medicine and Dentistry 17 9%
Veterinary Science and Veterinary Medicine 5 3%
Other 18 9%
Unknown 51 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2022.
All research outputs
#2,833,425
of 25,389,532 outputs
Outputs from PLOS ONE
#35,044
of 220,759 outputs
Outputs of similar age
#22,744
of 205,167 outputs
Outputs of similar age from PLOS ONE
#791
of 5,373 outputs
Altmetric has tracked 25,389,532 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 220,759 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.7. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 205,167 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 5,373 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.