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Identification and propagation of haploid human pluripotent stem cells

Overview of attention for article published in Nature Protocols, October 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
10 X users
facebook
1 Facebook page

Citations

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9 Dimensions

Readers on

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50 Mendeley
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Title
Identification and propagation of haploid human pluripotent stem cells
Published in
Nature Protocols, October 2016
DOI 10.1038/nprot.2016.145
Pubmed ID
Authors

Ido Sagi, Dieter Egli, Nissim Benvenisty

Abstract

Haploid human pluripotent stem cells (PSCs) integrate haploidy and pluripotency, providing a novel system for functional genomics and developmental research in humans. We have recently derived haploid human embryonic stem cells (ESCs) by parthenogenesis and demonstrated their wide differentiation potential and applicability for genetic screening. Because haploid cells can spontaneously become diploid, their enrichment at an early passage is key for successful derivation. In this protocol, we describe two methodologies, namely metaphase spread analysis and cell sorting, for the identification of haploid human cells within parthenogenetic ESC lines. The cell sorting approach also enables the isolation of haploid cells at low percentages, as well as the maintenance of highly enriched haploid ESC lines throughout passaging. The isolation of essentially pure populations of haploid human ESCs by this protocol requires basic PSC culture expertise and can be achieved within 4-6 weeks.

X Demographics

X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
Unknown 49 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 18%
Student > Bachelor 5 10%
Student > Ph. D. Student 5 10%
Professor > Associate Professor 4 8%
Other 3 6%
Other 8 16%
Unknown 16 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 28%
Agricultural and Biological Sciences 14 28%
Medicine and Dentistry 3 6%
Neuroscience 2 4%
Psychology 1 2%
Other 0 0%
Unknown 16 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2017.
All research outputs
#1,632,826
of 22,687,320 outputs
Outputs from Nature Protocols
#591
of 2,727 outputs
Outputs of similar age
#31,625
of 315,463 outputs
Outputs of similar age from Nature Protocols
#12
of 43 outputs
Altmetric has tracked 22,687,320 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,727 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,463 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.