| Title |
Fourier Transform Infrared Spectroscopic Imaging Parameters Describing Acid Phosphate Substitution in Biologic Hydroxyapatite
|
|---|---|
| Published in |
Calcified Tissue International, February 2013
|
| DOI | 10.1007/s00223-013-9695-9 |
| Pubmed ID | |
| Authors |
Lyudmila Spevak, Carol R. Flach, Tracey Hunter, Richard Mendelsohn, Adele Boskey |
| Abstract |
Acid phosphate substitution into mineralized tissues is an important determinant of their mechanical properties and their response to treatment. This study identifies and validates Fourier transform infrared spectroscopic imaging (FTIRI) spectral parameters that provide information on the acid phosphate (HPO4) substitution into hydroxyapatite in developing mineralized tissues. Curve fitting and Fourier self-deconvolution were used to identify subband positions in model compounds (with and without HPO4). The intensity of subbands at 1127 and 1110 cm(-1) correlated with the acid phosphate content in these models. Peak height ratios of these subbands to the ν3 vibration at 1096 cm(-1) found in stoichiometric apatite were evaluated in the model compounds and mixtures thereof. FTIRI spectra of bones and teeth at different developmental ages were analyzed using these spectral parameters. Factor analysis (a chemometric technique) was also conducted on the tissue samples and resulted in factor loadings with spectral features corresponding to the HPO4 vibrations described above. Images of both factor correlation coefficients and the peak height ratios 1127/1096 and 1112/1096 cm(-1) demonstrated higher acid phosphate content in younger vs. more mature regions in the same specimen. Maps of the distribution of acid phosphate content will be useful for characterizing the extent of new bone formation, the areas of potential decreased strength, and the effects of therapies such as those used in metabolic bone diseases (osteoporosis, chronic kidney disease) on mineral composition. Because of the wider range of values obtained with the 1127/1096 cm(-1) parameter compared to the 1110/1096 cm(-1) parameter and the smaller scatter in the slope, it is suggested that this ratio should be the parameter of choice. |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 73 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 26% |
| Other | 9 | 12% |
| Student > Master | 8 | 11% |
| Researcher | 7 | 10% |
| Student > Bachelor | 3 | 4% |
| Other | 11 | 15% |
| Unknown | 16 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 13 | 18% |
| Materials Science | 10 | 14% |
| Medicine and Dentistry | 8 | 11% |
| Chemistry | 7 | 10% |
| Agricultural and Biological Sciences | 3 | 4% |
| Other | 11 | 15% |
| Unknown | 21 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2013.
All research outputs
#15,265,264
of 22,699,621 outputs
Outputs from Calcified Tissue International
#1,335
of 1,756 outputs
Outputs of similar age
#182,432
of 282,923 outputs
Outputs of similar age from Calcified Tissue International
#7
of 16 outputs
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