Title |
Isolation and Purification of an Early Pregnancy Factor–like Molecule from Culture Supernatants Obtained from Lymphocytes of Pregnant Women: II. Identification of the Molecule as a FC-Receptor–like Molecule: A Preliminary Report
|
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Published in |
Journal of Assisted Reproduction and Genetics, November 1998
|
DOI | 10.1023/a:1020389512677 |
Pubmed ID | |
Authors |
Clara Aranha, Arvind Bordekar, Savitri Shahani |
Abstract |
Early pregnancy factor (EPF)-like activity from culture supernatants obtained from stimulated lymphocytes of pregnant women was characterized and identified. The enzyme-linked immunosorbent assay depending on the presence of "Fc" receptors on bovine spermatozoa was used to identify the EPF-like molecule purified by gel filtration and reverse-phase high-performance liquid chromatography. The results indicated that the crude lymphocyte culture supernatant, the EPF-positive G IV fraction obtained on gel filtration, and the EPF-positive reverse-phase high-performance liquid chromatography protein readily bound with the different concentrations of aggregated human gamma-globulin in a manner similar to that in which the standard control of aggregated human gamma-globulin binds to the bovine spermatozoa. EPF-like activity synthesized and secreted by lymphocytes during pregnancy may be a Fc-receptor-like molecule. |
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