| Title |
Drought-inducible expression of Hv-miR827 enhances drought tolerance in transgenic barley
|
|---|---|
| Published in |
Functional & Integrative Genomics, October 2016
|
| DOI | 10.1007/s10142-016-0526-8 |
| Pubmed ID | |
| Authors |
Jannatul Ferdous, Ryan Whitford, Martin Nguyen, Chris Brien, Peter Langridge, Penny J. Tricker |
| Abstract |
Drought is one of the major abiotic stresses reducing crop yield. Since the discovery of plant microRNAs (miRNAs), considerable progress has been made in clarifying their role in plant responses to abiotic stresses, including drought. miR827 was previously reported to confer drought tolerance in transgenic Arabidopsis. We examined barley (Hordeum vulgare L. 'Golden Promise') plants over-expressing miR827 for plant performance under drought. Transgenic plants constitutively expressing CaMV-35S::Ath-miR827 and drought-inducible Zm-Rab17::Hv-miR827 were phenotyped by non-destructive imaging for growth and whole plant water use efficiency (WUEwp). We observed that the growth, WUEwp, time to anthesis and grain weight of transgenic barley plants expressing CaMV-35S::Ath-miR827 were negatively affected in both well-watered and drought-treated growing conditions compared with the wild-type plants. In contrast, transgenic plants over-expressing Zm-Rab17::Hv-miR827 showed improved WUEwp with no growth or reproductive timing change compared with the wild-type plants. The recovery of Zm-Rab17::Hv-miR827 over-expressing plants also improved following severe drought stress. Our results suggest that Hv-miR827 has the potential to improve the performance of barley under drought and that the choice of promoter to control the timing and specificity of miRNA expression is critical. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 53 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 34% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Master | 4 | 8% |
| Student > Bachelor | 2 | 4% |
| Student > Doctoral Student | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 13 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 42% |
| Biochemistry, Genetics and Molecular Biology | 12 | 23% |
| Mathematics | 1 | 2% |
| Social Sciences | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 16 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2017.
All research outputs
#2,890,881
of 22,896,955 outputs
Outputs from Functional & Integrative Genomics
#10
of 511 outputs
Outputs of similar age
#51,395
of 320,038 outputs
Outputs of similar age from Functional & Integrative Genomics
#1
of 13 outputs
Altmetric has tracked 22,896,955 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 511 research outputs from this source. They receive a mean Attention Score of 2.6. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.