| Title |
Comparison of the Gut Microbe Profiles and Numbers Between Patients with Liver Cirrhosis and Healthy Individuals
|
|---|---|
| Published in |
Current Microbiology, April 2012
|
| DOI | 10.1007/s00284-012-0105-8 |
| Pubmed ID | |
| Authors |
Jianjun Liu, Dachang Wu, Ayaz Ahmed, Xinli Li, Yufang Ma, Li Tang, Dianjun Mo, Yue Ma, Yi Xin |
| Abstract |
Human liver was closely associated with gut through various biological mechanisms, such as bacterium-gut interactions. Alterations of gut microbiota seemed to play an important role in induction and promotion of liver damage progression. The aim of this study was to characterize the gut microbiota in liver cirrhosis patients and assess whether there are alterations in the diversity and similarity of intestinal flora in cirrhotic patients when compared with healthy individuals. PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers targeting V3 region of the 16S rRNA gene was employed to characterize the overall intestinal microbiota composition, and some excised gel bands were cloned for sequencing. Real-time PCR was further utilized to quantitatively analyze the subpopulation of microbiota using group-specific primers targeting the Enterobacteriaceae, Enterococcus and Bifidobacterium genus. The DGGE profiles of two groups demonstrated significant differences between cirrhotic and healthy groups (P < 0.05). While real-time PCR revealed significant increase of Enterobacteriaceae and Enterococcus (P < 0.05) in the cirrhotic group compared with the healthy group. The ratio of Bifidobacterium genus and Enterobacteriaceae decreased in the cirrhotic patients group, but no statistical significance. This study revealed strong relationship between alterations of gut microbiota and liver cirrhosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 2% |
| United Kingdom | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 60 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 21% |
| Student > Ph. D. Student | 11 | 17% |
| Student > Master | 9 | 14% |
| Student > Bachelor | 8 | 13% |
| Other | 3 | 5% |
| Other | 9 | 14% |
| Unknown | 10 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 17 | 27% |
| Agricultural and Biological Sciences | 16 | 25% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Immunology and Microbiology | 4 | 6% |
| Environmental Science | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 17 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2012.
All research outputs
#20,185,720
of 22,701,287 outputs
Outputs from Current Microbiology
#1,903
of 2,398 outputs
Outputs of similar age
#146,083
of 161,386 outputs
Outputs of similar age from Current Microbiology
#15
of 16 outputs
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So far Altmetric has tracked 2,398 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.