| Title |
Action of two phospholipases A2 purified from Bothrops alternatus snake venom on macrophages
|
|---|---|
| Published in |
Biochemistry, February 2013
|
| DOI | 10.1134/s0006297913020089 |
| Pubmed ID | |
| Authors |
S. S. Setúbal, A. S. Pontes, J. L. Furtado, C. V. Xavier, F. L. Silva, A. M. Kayano, L. F. M. Izidoro, A. M. Soares, L. A. Calderon, R. G. Stábeli, J. P. Zuliani |
| Abstract |
The in vitro effects of BaltTX-I, a catalytically inactive Lys49 variant of phospholipase A2 (PLA2), and BaltTX-II, an Asp49 catalytically active PLA2 isolated from Bothrops alternatus snake venom, on thioglycollate-elicited macrophages (TG-macrophages) were investigated. At non-cytotoxic concentrations, the secretory PLA2 BaltTX-I but not BaltTX-II stimulated complement receptor-mediated phagocytosis. Pharmacological treatment of TG-macrophages with staurosporine, a protein kinase C (PKC) inhibitor, showed that this kinase is involved in the increase of serum-opsonized zymosan phagocytosis induced by BaltTX-I but not BaltTX-II secretory PLA2, suggesting that PKC may be involved in the stimulatory effect of this toxin in serum-opsonized zymosan phagocytosis. Moreover, BaltTX-I and -II induced superoxide production by TG-macrophages. This superoxide production stimulated by both PLA2s was abolished after treatment of cells with staurosporine, indicating that PKC is an important signaling pathway for the production of this radical. Our experiments showed that, at non-cytotoxic concentrations, BaltTX-I may upregulate phagocytosis via complement receptors, and that both toxins upregulated the respiratory burst in TG-macrophages. |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 45 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 22% |
| Student > Master | 8 | 17% |
| Student > Doctoral Student | 6 | 13% |
| Researcher | 4 | 9% |
| Student > Ph. D. Student | 4 | 9% |
| Other | 7 | 15% |
| Unknown | 7 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 46% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Immunology and Microbiology | 3 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Chemistry | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 9 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2013.
All research outputs
#22,758,309
of 25,373,627 outputs
Outputs from Biochemistry
#21,449
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Outputs of similar age
#180,566
of 204,117 outputs
Outputs of similar age from Biochemistry
#69
of 86 outputs
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