| Title |
Isolation, Culture, and Identification of Amniotic Fluid-Derived Mesenchymal Stem Cells
|
|---|---|
| Published in |
Cell Biochemistry and Biophysics, March 2013
|
| DOI | 10.1007/s12013-013-9558-z |
| Pubmed ID | |
| Authors |
Xuetao Fei, Shan Jiang, Song Zhang, Yigang Li, Junbo Ge, Ben He, Steven Goldstein, George Ruiz |
| Abstract |
Amniotic fluid-derived mesenchymal stem cells (AF-MSC) are newly described, excellent seed cells that have good differentiation capability and are convenient to obtain. However, it is important to develop a method to isolate and culture AF-MSC efficiently. Amniotic fluid samples were obtained from rabbits and the adherence method was used for AF-MSC culture. Flow cytometry, western blot, and immunofluorescence studies were used to analyze the phenotypic characteristics of the cultured AF-MSC. Amniotic fluid-derived mesenchymal stem cells were successfully isolated and cultured from amniotic fluid. Flow cytometric analysis demonstrated that these cells expressed CD29 and CD44, while they did not express CD34. The expression of transcription factor Oct-4 was confirmed by western blot and immunofluorescence analysis. Using the adherence method, we developed a successful, reproducible protocol for the isolation of AF-MSC from amniotic fluid. The results of our phenotypic analysis revealed that the AF-MSC isolated in the present study were multipotent cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 2% |
| Unknown | 46 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 21% |
| Student > Master | 8 | 17% |
| Student > Bachelor | 4 | 9% |
| Researcher | 4 | 9% |
| Student > Doctoral Student | 3 | 6% |
| Other | 7 | 15% |
| Unknown | 11 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 10 | 21% |
| Agricultural and Biological Sciences | 9 | 19% |
| Biochemistry, Genetics and Molecular Biology | 6 | 13% |
| Engineering | 3 | 6% |
| Social Sciences | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 13 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2013.
All research outputs
#17,682,134
of 22,701,287 outputs
Outputs from Cell Biochemistry and Biophysics
#423
of 910 outputs
Outputs of similar age
#143,376
of 197,433 outputs
Outputs of similar age from Cell Biochemistry and Biophysics
#20
of 24 outputs
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