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Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis

Overview of attention for article published in Development (09501991), March 2013
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Title
Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis
Published in
Development (09501991), March 2013
DOI 10.1242/dev.093583
Pubmed ID
Authors

Katarzyna Koltowska, Holger Apitz, Despina Stamataki, Elizabeth M. A. Hirst, Heather Verkade, Iris Salecker, Elke A. Ober

Abstract

Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G1 phase progression. Gene deletion/replacement experiments in Drosophila show that Ssrp1b, Ssrp1a and N-terminal Ssrp1a, equivalent to the yeast homologue Pob3, can substitute Drosophila Ssrp function. These data suggest that (1) Ssrp1b does not compensate for Ssrp1a loss in the zebrafish embryo, probably owing to insufficient expression levels, and (2) despite fundamental structural differences, the mechanisms mediating DNA accessibility by FACT are conserved between yeast and metazoans. We propose that the essential functions of Ssrp1a in DNA replication and gene transcription, together with its dynamic spatiotemporal expression, ensure organ-specific differentiation and proportional growth, which are crucial for the forming embryo.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 7%
Unknown 26 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 36%
Researcher 10 36%
Professor > Associate Professor 2 7%
Student > Master 2 7%
Unknown 4 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 54%
Biochemistry, Genetics and Molecular Biology 6 21%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Design 1 4%
Unknown 5 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2013.
All research outputs
#16,072,027
of 25,411,814 outputs
Outputs from Development (09501991)
#7,482
of 9,475 outputs
Outputs of similar age
#124,119
of 210,290 outputs
Outputs of similar age from Development (09501991)
#74
of 103 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,475 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.8. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 210,290 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 103 others from the same source and published within six weeks on either side of this one. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.