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Viral infection modulation and neutralization by camelid nanobodies

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, March 2013
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Title
Viral infection modulation and neutralization by camelid nanobodies
Published in
Proceedings of the National Academy of Sciences of the United States of America, March 2013
DOI 10.1073/pnas.1301336110
Pubmed ID
Authors

Aline Desmyter, Carine Farenc, Jennifer Mahony, Silvia Spinelli, Cecilia Bebeacua, Stéphanie Blangy, David Veesler, Douwe van Sinderen, Christian Cambillau

Abstract

Lactococcal phages belong to a large family of Siphoviridae and infect Lactococcus lactis, a gram-positive bacterium used in commercial dairy fermentations. These phages are believed to recognize and bind specifically to pellicle polysaccharides covering the entire bacterium. The phage TP901-1 baseplate, located at the tip of the tail, harbors 18 trimeric receptor binding proteins (RBPs) promoting adhesion to a specific lactococcal strain. Phage TP901-1 adhesion does not require major conformational changes or Ca(2+), which contrasts other lactococcal phages. Here, we produced and characterized llama nanobodies raised against the purified baseplate and the Tal protein of phage TP901-1 as tools to dissect the molecular determinants of phage TP901-1 infection. Using a set of complementary techniques, surface plasmon resonance, EM, and X-ray crystallography in a hybrid approach, we identified binders to the three components of the baseplate, analyzed their affinity for their targets, and determined their epitopes as well as their functional impact on TP901-1 phage infectivity. We determined the X-ray structures of three nanobodies in complex with the RBP. Two of them bind to the saccharide binding site of the RBP and are able to fully neutralize TP901-1 phage infectivity, even after 15 passages. These results provide clear evidence for a practical use of nanobodies in circumventing lactococcal phages viral infection in dairy fermentation.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 68 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 28%
Student > Ph. D. Student 14 21%
Student > Bachelor 6 9%
Student > Master 4 6%
Student > Doctoral Student 3 4%
Other 11 16%
Unknown 11 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 31%
Agricultural and Biological Sciences 16 24%
Immunology and Microbiology 4 6%
Veterinary Science and Veterinary Medicine 3 4%
Medicine and Dentistry 3 4%
Other 8 12%
Unknown 13 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2014.
All research outputs
#16,741,542
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#91,769
of 101,438 outputs
Outputs of similar age
#128,516
of 201,290 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#819
of 986 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 201,290 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 986 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.