| Title |
Antigenic characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in India, 2006-2015
|
|---|---|
| Published in |
Archives of Virology, November 2016
|
| DOI | 10.1007/s00705-016-3134-y |
| Pubmed ID | |
| Authors |
Sudipta Bhat, Shanmugasundaram Nagarajan, Manoj Kumar, Harshad V. Murugkar, Semmannan Kalaiyarasu, Govindarajulu Venkatesh, Chakradhar Tosh |
| Abstract |
Highly pathogenic avian influenza (HPAI) is a major health concern worldwide. In this study, we focused on antigenic analysis of HPAI H5N1 viruses isolated from poultry in India between 2006 and 2015 comprising 25 isolates from four phylogenetic clades 2.2 (1 isolate), 2.2.2.1 (1 isolate), 2.3.2.1a (17 isolates) and 2.3.2.1c (6 isolates). Seven H5N1 isolates from all four clades were selected for production of chicken antiserum, and antigenic analysis was carried out by hemagglutination inhibition (HI) assay. HI data indicated antigenic divergence (6-21 fold reduction in cross-reactivity) between the two recently emerged clades 2.3.2.1a and 2.3.2.1c. These two clades are highly divergent (21-128 fold reduction in HI titre) from the earlier clades 2.2 /2.2.2.1 isolated in India. However, a maximum of 2-fold and 4-fold reduction in cross-reactivity was observed within the isolates of homologous clades 2.3.2.1c and 2.3.2.1a, respectively. The molecular basis of inter-clade antigenic divergence was examined in the haemagglutinin (HA) antigenic sites of the H5N1 virus. Amino acid changes at 8 HA antigenic sites were observed between clades 2.3.2.1a and 2.3.2.1c, whereas 20-23 substitutions were observed between clades 2.3.2.1a/2.3.2.1c and 2.2/2.2.2.1. Therefore, a systematic analysis of antigenic drift of the contemporary field isolates is a pre-requisite for determining the suitable strain(s) for vaccine candidature. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 29% |
| Other | 2 | 10% |
| Student > Postgraduate | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 19% |
| Veterinary Science and Veterinary Medicine | 2 | 10% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Medicine and Dentistry | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 7 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2016.
All research outputs
#20,351,881
of 22,899,952 outputs
Outputs from Archives of Virology
#3,401
of 4,193 outputs
Outputs of similar age
#269,113
of 311,569 outputs
Outputs of similar age from Archives of Virology
#26
of 35 outputs
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