| Title |
Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, November 2016
|
| DOI | 10.1007/s00216-016-0015-x |
| Pubmed ID | |
| Authors |
Yasuo Shimizu, Motoyasu Satou, Keitaro Hayashi, Yusuke Nakamura, Mio Fujimaki, Yasuhiro Horibata, Hiromi Ando, Taiji Watanabe, Taichi Shiobara, Kazuyuki Chibana, Akihiro Takemasa, Hiroyuki Sugimoto, Naohiko Anzai, Yoshiki Ishii |
| Abstract |
Induced pluripotent stem cells (iPSCs) are opening up new possibilities for medicine. Understanding the regulation of iPSC biology is important when attempting to apply these cells to disease models or therapy. Changes of lipid metabolism in iPSCs were investigated by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF-IMS). Analysis revealed changes of the intensity and distribution of peaks at m/z 782.5 and 798.5 in iPSC colonies during spontaneous differentiation. Two phosphatidylcholines (PCs) were identified: C44H81NO8P, PC(36:4)[M+H]+ at m/z 782.5 and C42H82NO8P, PC(34:1)[M+K]+ at m/z 798.5. The intensity of PC(36:4) showed an inverse relation between undifferentiated and differentiated iPSC colonies. PC(34:1) displayed a diffuse distribution in undifferentiated iPSC colonies, while it showed a concentric distribution in differentiated iPSC colonies, and was localized at the border of the differentiated and undifferentiated areas or the border between undifferentiated iPSC and feeder cells. These findings suggested that the distribution of lipids changes during the growth and differentiation of iPSCs and that MALDI-TOF-IMS was useful for analyzing these changes. PC(36:4) might play a role in maintaining pluripotency, while PC(34:1) might play a role in the differentiation and spread of iPSCs. Graphical Abstract MALDI Imaging for phosphatidylcholine distribution changes during sponteneous differentiaton of induced pluiripotent stem cells colonies. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 50% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 50% |
| Scientists | 2 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 27% |
| Researcher | 4 | 15% |
| Student > Bachelor | 2 | 8% |
| Professor > Associate Professor | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 4 | 15% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 7 | 27% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Agricultural and Biological Sciences | 3 | 12% |
| Medicine and Dentistry | 2 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 6 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2017.
All research outputs
#16,048,009
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,978
of 9,619 outputs
Outputs of similar age
#186,296
of 317,470 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#47
of 195 outputs
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