| Title |
ADAMTS-2 functions as anti-angiogenic and anti-tumoral molecule independently of its catalytic activity
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, June 2010
|
| DOI | 10.1007/s00018-010-0431-6 |
| Pubmed ID | |
| Authors |
J. Dubail, F. Kesteloot, C. Deroanne, P. Motte, V. Lambert, J.-M. Rakic, C. Lapière, B. Nusgens, A. Colige |
| Abstract |
ADAMTS-2 is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. We demonstrate that recombinant ADAMTS-2 is also able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. Dephosphorylation of Erk1/2 and MLC largely precedes the ADAMTS-2 induced morphological alterations. In 3-D culture models, ADAMTS-2 strongly reduced branching of capillary-like structures formed by endothelial cells and their long-term maintenance and inhibited vessels formation in embryoid bodies (EB). Growth and vascularization of tumors formed in nude mice by HEK 293-EBNA cells expressing ADAMTS-2 were drastically reduced. A similar anti-tumoral activity was observed when using cells expressing recombinant deleted forms of ADAMTS-2, including catalytically inactive enzyme. Nucleolin, a nuclear protein also found to be associated with the cell membrane, was identified as a potential receptor mediating the antiangiogenic properties of ADAMTS-2. |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Spain | 1 | 2% |
| Belgium | 1 | 2% |
| Unknown | 49 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 23% |
| Researcher | 10 | 19% |
| Student > Bachelor | 7 | 13% |
| Student > Master | 5 | 10% |
| Student > Doctoral Student | 4 | 8% |
| Other | 8 | 15% |
| Unknown | 6 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 35% |
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Medicine and Dentistry | 6 | 12% |
| Neuroscience | 3 | 6% |
| Arts and Humanities | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 8 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2010.
All research outputs
#7,591,533
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#1,602
of 4,151 outputs
Outputs of similar age
#33,766
of 95,908 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#11
of 31 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 4,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 95,908 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.