Title |
Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks
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Published in |
Chromosome Research, November 2006
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DOI | 10.1007/s10577-006-1086-x |
Pubmed ID | |
Authors |
Heiner Albiez, Marion Cremer, Cinzia Tiberi, Lorella Vecchio, Lothar Schermelleh, Sandra Dittrich, Katrin Küpper, Boris Joffe, Tobias Thormeyer, Johann von Hase, Siwei Yang, Karl Rohr, Heinrich Leonhardt, Irina Solovei, Christoph Cremer, Stanislav Fakan, Thomas Cremer |
Abstract |
In spite of strong evidence that the nucleus is a highly organized organelle, a consensus on basic principles of the global nuclear architecture has not so far been achieved. The chromosome territory-interchromatin compartment (CT-IC) model postulates an IC which expands between chromatin domains both in the interior and the periphery of CT. Other models, however, dispute the existence of the IC and claim that numerous chromatin loops expand between and within CTs. The present study was undertaken to resolve these conflicting views. (1) We demonstrate that most chromatin exists in the form of higher-order chromatin domains with a compaction level at least 10 times above the level of extended 30 nm chromatin fibers. A similar compaction level was obtained in a detailed analysis of a particularly gene-dense chromosome region on HSA 11, which often expanded from its CT as a finger-like chromatin protrusion. (2) We further applied an approach which allows the experimental manipulation of both chromatin condensation and the width of IC channels in a fully reversible manner. These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
United Kingdom | 3 | 1% |
France | 3 | 1% |
Germany | 2 | <1% |
Poland | 2 | <1% |
Korea, Republic of | 1 | <1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Portugal | 1 | <1% |
Other | 2 | <1% |
Unknown | 183 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 57 | 28% |
Student > Ph. D. Student | 54 | 27% |
Student > Bachelor | 15 | 7% |
Student > Doctoral Student | 13 | 6% |
Student > Master | 13 | 6% |
Other | 30 | 15% |
Unknown | 20 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 109 | 54% |
Biochemistry, Genetics and Molecular Biology | 48 | 24% |
Physics and Astronomy | 9 | 4% |
Computer Science | 3 | 1% |
Engineering | 3 | 1% |
Other | 9 | 4% |
Unknown | 21 | 10% |