| Chapter title |
Evaluating the Delivery of Proteins to the Cytosol of Mammalian Cells.
|
|---|---|
| Chapter number | 14 |
| Book title |
Cancer Gene Networks
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6539-7_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6537-3, 978-1-4939-6539-7
|
| Authors |
Andrea L. J. Marschall, Congcong Zhang, Stefan Dübel, Marschall, Andrea L. J., Zhang, Congcong, Dübel, Stefan |
| Editors |
Usha Kasid, Robert Clarke |
| Abstract |
Delivery of proteins to the cytosol of living cells is a promising research tool. Delivery of antibodies in particular bears exciting applications such as in vivo tracking of proteins at endogenous expression levels or interference with cellular processes. In spite of the large number of methods published for protein delivery, successful applications so far are rare. A possible explanation for this is a vast overestimation of the delivery efficiency due to the use of inappropriate detection methods and/or unsuitable positive controls for cytosolic delivery. Therefore, we provide strategies for unequivocally detecting cytoplasmic protein delivery and quantifying protein transformation efficiency. Finally, we present a protocol for efficient protein delivery to the cytosol validated using these methods. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Bachelor | 2 | 18% |
| Other | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Neuroscience | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |