| Title |
Arsenic Induces Apoptosis of Human Umbilical Vein Endothelial Cells Through Mitochondrial Pathways
|
|---|---|
| Published in |
Cardiovascular Toxicology, May 2010
|
| DOI | 10.1007/s12012-010-9073-z |
| Pubmed ID | |
| Authors |
Yanfen Shi, Yudan Wei, Shanshan Qu, Yang Wang, Yulin Li, Ronggui Li |
| Abstract |
To clarify the molecular mechanisms through which arsenic causes injuries to blood vessels, we analyzed the effects of sodium arsenite (NaAsO(2)) on the apoptosis of human umbilical vein endothelial cells (HUVECs), mitochondrial membrane potential (Delta Psi m), intracellular reactive oxygen species (ROS), and the expression of the related genes. HUVECs apoptosis increased and Delta Psi m decreased in a dose-dependent manner following arsenic treatment. Intracellular ROS showed 2 phase alterations: a slight decrease with low levels of arsenic (5 and 10 microM) treatment; but a sharp increase at higher concentrations (>or=20 microM). The arsenic-induced cell apoptosis and intracellular ROS were blocked by the addition of the antioxidant N-acetyl-L-cysteine (NAC). The mRNAs of superoxide dismutase 2 (SOD2) and NAD(P)H:quinone oxidoreductase 1 (NQO1) increased strikingly when cells were treated with a low concentration of NaAsO(2) (5 microM) and the level of induction was decreased with higher concentrations of arsenic treatment. Based on the results, we suggest that the decrease of Delta Psi m caused by arsenic and the resulting cell apoptosis may contribute to the injuries of blood vessel in arsenism. The decrease in intracellular ROS and the increase in SOD2 and NQO1 expressions observed when HUVECs were treated with low concentration of NaAsO(2), suggest the role of the two enzymes in protecting HUVECs from injuries of arsenic exposure. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 6% |
| China | 1 | 6% |
| Unknown | 15 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 41% |
| Student > Master | 3 | 18% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Doctoral Student | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 41% |
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Medicine and Dentistry | 2 | 12% |
| Nursing and Health Professions | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2010.
All research outputs
#20,187,333
of 22,703,044 outputs
Outputs from Cardiovascular Toxicology
#204
of 279 outputs
Outputs of similar age
#90,492
of 94,968 outputs
Outputs of similar age from Cardiovascular Toxicology
#4
of 4 outputs
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