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Assessment of Intrathecal Free Light Chain Synthesis: Comparison of Different Quantitative Methods with the Detection of Oligoclonal Free Light Chains by Isoelectric Focusing and Affinity-Mediated…

Overview of attention for article published in PLOS ONE, November 2016
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Title
Assessment of Intrathecal Free Light Chain Synthesis: Comparison of Different Quantitative Methods with the Detection of Oligoclonal Free Light Chains by Isoelectric Focusing and Affinity-Mediated Immunoblotting
Published in
PLOS ONE, November 2016
DOI 10.1371/journal.pone.0166556
Pubmed ID
Authors

David Zeman, Pavlína Kušnierová, Zdeněk Švagera, František Všianský, Monika Byrtusová, Pavel Hradílek, Barbora Kurková, Olga Zapletalová, Vladimír Bartoš

Abstract

We aimed to compare various methods for free light chain (fLC) quantitation in cerebrospinal fluid (CSF) and serum and to determine whether quantitative CSF measurements could reliably predict intrathecal fLC synthesis. In addition, we wished to determine the relationship between free kappa and free lambda light chain concentrations in CSF and serum in various disease groups. We analysed 166 paired CSF and serum samples by at least one of the following methods: turbidimetry (Freelite™, SPAPLUS), nephelometry (N Latex FLC™, BN ProSpec), and two different (commercially available and in-house developed) sandwich ELISAs. The results were compared with oligoclonal fLC detected by affinity-mediated immunoblotting after isoelectric focusing. Although the correlations between quantitative methods were good, both proportional and systematic differences were discerned. However, no major differences were observed in the prediction of positive oligoclonal fLC test. Surprisingly, CSF free kappa/free lambda light chain ratios were lower than those in serum in about 75% of samples with negative oligoclonal fLC test. In about a half of patients with multiple sclerosis and clinically isolated syndrome, profoundly increased free kappa/free lambda light chain ratios were found in the CSF. Our results show that using appropriate method-specific cut-offs, different methods of CSF fLC quantitation can be used for the prediction of intrathecal fLC synthesis. The reason for unexpectedly low free kappa/free lambda light chain ratios in normal CSFs remains to be elucidated. Whereas CSF free kappa light chain concentration is increased in most patients with multiple sclerosis and clinically isolated syndrome, CSF free lambda light chain values show large interindividual variability in these patients and should be investigated further for possible immunopathological and prognostic significance.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 10%
Other 2 7%
Lecturer > Senior Lecturer 2 7%
Student > Postgraduate 2 7%
Student > Bachelor 2 7%
Other 7 23%
Unknown 12 40%
Readers by discipline Count As %
Medicine and Dentistry 6 20%
Business, Management and Accounting 2 7%
Biochemistry, Genetics and Molecular Biology 2 7%
Neuroscience 2 7%
Agricultural and Biological Sciences 1 3%
Other 4 13%
Unknown 13 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 November 2016.
All research outputs
#15,393,913
of 22,901,818 outputs
Outputs from PLOS ONE
#131,391
of 195,245 outputs
Outputs of similar age
#193,461
of 306,451 outputs
Outputs of similar age from PLOS ONE
#2,524
of 3,928 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 195,245 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 3,928 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.