| Title |
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
|
|---|---|
| Published in |
Genome Biology, June 2002
|
| DOI | 10.1186/gb-2002-3-7-research0034 |
| Pubmed ID | |
| Authors |
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman |
| Abstract |
Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large dynamic range of this methodology, the requirements for a proper internal control gene for normalization have become increasingly stringent. Although housekeeping gene expression has been reported to vary considerably, no systematic survey has properly determined the errors related to the common practice of using only one control gene, nor presented an adequate way of working around this problem. |
X Demographics
The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 25% |
| United Kingdom | 4 | 20% |
| Canada | 2 | 10% |
| India | 1 | 5% |
| Switzerland | 1 | 5% |
| Japan | 1 | 5% |
| Spain | 1 | 5% |
| Unknown | 5 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 11 | 55% |
| Scientists | 8 | 40% |
| Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 8,350 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 87 | 1% |
| Brazil | 55 | <1% |
| United Kingdom | 54 | <1% |
| Germany | 47 | <1% |
| Belgium | 26 | <1% |
| Spain | 22 | <1% |
| Mexico | 21 | <1% |
| France | 20 | <1% |
| Portugal | 19 | <1% |
| Other | 222 | 3% |
| Unknown | 7777 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2105 | 25% |
| Researcher | 1706 | 20% |
| Student > Master | 1134 | 14% |
| Student > Bachelor | 709 | 8% |
| Student > Doctoral Student | 519 | 6% |
| Other | 1151 | 14% |
| Unknown | 1026 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3904 | 47% |
| Biochemistry, Genetics and Molecular Biology | 1439 | 17% |
| Medicine and Dentistry | 641 | 8% |
| Neuroscience | 175 | 2% |
| Immunology and Microbiology | 163 | 2% |
| Other | 757 | 9% |
| Unknown | 1271 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 76. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2024.
All research outputs
#570,466
of 25,837,817 outputs
Outputs from Genome Biology
#336
of 4,513 outputs
Outputs of similar age
#392
of 49,380 outputs
Outputs of similar age from Genome Biology
#2
of 24 outputs
Altmetric has tracked 25,837,817 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,513 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.7. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 49,380 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.