| Title |
Adipocyte STAT5 deficiency promotes adiposity and impairs lipid mobilisation in mice
|
|---|---|
| Published in |
Diabetologia, November 2016
|
| DOI | 10.1007/s00125-016-4152-8 |
| Pubmed ID | |
| Authors |
Doris Kaltenecker, Kristina M. Mueller, Pia Benedikt, Ursula Feiler, Madeleine Themanns, Michaela Schlederer, Lukas Kenner, Martina Schweiger, Guenter Haemmerle, Richard Moriggl |
| Abstract |
Dysfunction of lipid metabolism in white adipose tissue can substantially interfere with health and quality of life, for example in obesity and associated metabolic diseases. Therefore, it is important to characterise pathways that regulate lipid handling in adipocytes and determine how they affect metabolic homeostasis. Components of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway are involved in adipocyte physiology and pathophysiology. However, the exact physiological importance of the STAT family member STAT5 in white adipose tissue is yet to be determined. Here, we aimed to delineate adipocyte STAT5 functions in the context of lipid metabolism in white adipose tissue. We generated an adipocyte specific knockout of Stat5 in mice using the Adipoq-Cre recombinase transgene followed by in vivo and in vitro biochemical and molecular studies. Adipocyte-specific deletion of Stat5 resulted in increased adiposity, while insulin resistance and gluconeogenic capacity was decreased, indicating that glucose metabolism can be improved by interfering with adipose STAT5 function. Basal lipolysis and fasting-induced lipid mobilisation were diminished upon STAT5 deficiency, which coincided with reduced levels of the rate-limiting lipase of triacylglycerol hydrolysis, adipose triglyceride lipase (ATGL, encoded by Pnpla2) and its coactivator comparative gene identification 58 (CGI-58). In a mechanistic analysis, we identified a functional STAT5 response element within the Pnpla2 promoter, indicating that Pnpla2 is transcriptionally regulated by STAT5. Our findings reveal an essential role for STAT5 in maintaining lipid homeostasis in white adipose tissue and provide a rationale for future studies into the potential of STAT5 manipulation to improve outcomes in metabolic diseases. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 2 | 18% |
| United States | 2 | 18% |
| Australia | 1 | 9% |
| United Kingdom | 1 | 9% |
| France | 1 | 9% |
| Germany | 1 | 9% |
| Unknown | 3 | 27% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 6 | 55% |
| Members of the public | 5 | 45% |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 57 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 21% |
| Researcher | 8 | 14% |
| Student > Master | 7 | 12% |
| Student > Bachelor | 4 | 7% |
| Professor | 4 | 7% |
| Other | 8 | 14% |
| Unknown | 14 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 28% |
| Agricultural and Biological Sciences | 10 | 18% |
| Medicine and Dentistry | 6 | 11% |
| Sports and Recreations | 2 | 4% |
| Unspecified | 1 | 2% |
| Other | 5 | 9% |
| Unknown | 17 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2017.
All research outputs
#1,506,853
of 22,901,818 outputs
Outputs from Diabetologia
#822
of 5,040 outputs
Outputs of similar age
#32,949
of 417,510 outputs
Outputs of similar age from Diabetologia
#24
of 73 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,040 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.7. This one has done well, scoring higher than 83% of its peers.
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We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.