Title |
Capturing Excited States in the Fast‐Intermediate Exchange Limit in Biological Systems Using 1H NMR Spectroscopy
|
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Published in |
Angewandte Chemie. International Edition, November 2016
|
DOI | 10.1002/anie.201609102 |
Pubmed ID | |
Authors |
Emilie Steiner, Judith Schlagnitweit, Patrik Lundström, Katja Petzold |
Abstract |
Changes in molecular structure are essential for the function of biomolecules. Characterization of these structural fluctuations can illuminate alternative states and help in correlating structure to function. NMR relaxation dispersion (RD) is currently the only method for detecting these alternative, high-energy states. In this study, we present a versatile (1) H R1ρ RD experiment that not only extends the exchange timescales at least three times beyond the rate limits of (13) C/(15) N R1ρ and ten times for CPMG experiments, but also makes use of easily accessible probes, thus allowing a general description of biologically important excited states. This technique can be used to extract chemical shifts for the structural characterization of excited states and to elucidate complex excited states. |
X Demographics
Geographical breakdown
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Germany | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
Unknown | 50 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 22% |
Researcher | 8 | 16% |
Student > Doctoral Student | 4 | 8% |
Other | 3 | 6% |
Student > Postgraduate | 3 | 6% |
Other | 10 | 20% |
Unknown | 12 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 18 | 35% |
Chemistry | 12 | 24% |
Agricultural and Biological Sciences | 3 | 6% |
Physics and Astronomy | 1 | 2% |
Unspecified | 1 | 2% |
Other | 2 | 4% |
Unknown | 14 | 27% |