| Title |
Insulitis in human diabetes: a histological evaluation of donor pancreases
|
|---|---|
| Published in |
Diabetologia, October 2016
|
| DOI | 10.1007/s00125-016-4140-z |
| Pubmed ID | |
| Authors |
Marcus Lundberg, Peter Seiron, Sofie Ingvast, Olle Korsgren, Oskar Skog |
| Abstract |
According to the consensus criteria developed for type 1 diabetes, an individual can be diagnosed with insulitis when ≥ 15 CD45(+) cells are found within the parenchyma or in the islet-exocrine interface in ≥ 3 islets. The aim of this study was to determine the frequency of individuals with type 2 diabetes fulfilling these criteria with reference to non-diabetic and type 1 diabetic individuals. Insulitis was determined by examining CD45(+) cells in the pancreases of 50, 13 and 44 organ donors with type 2 diabetes, type 1 diabetes and no diabetes, respectively. CD3(+) cells (T cells) infiltrating the islets were evaluated in insulitic donors. In insulitic donors with type 2 diabetes, the pancreases were characterised according to the presence of CD68 (macrophages), myeloperoxidase (MPO; neutrophils), CD3, CD20 (B cells) and HLA class I hyperstained islets. In all type 2 diabetic donors, potential correlations of insulitis with dynamic glucose-stimulated insulin secretion in vitro or age, BMI, HbA1c or autoantibody positivity were examined. Overall, 28% of the type 2 diabetic donors fulfilled the consensus criteria for insulitis developed for type 1 diabetes. Of the type 1 diabetic donors, 31% fulfilled the criteria. None of the non-diabetic donors met the criteria. Only type 1 diabetic donors had ≥ 15 CD3(+) cells in ≥ 3 islets. Type 2 diabetic donors with insulitis also had a substantial number of CD45(+) cells in the exocrine parenchyma. Macrophages constituted the largest fraction of CD45(+) cells, followed by neutrophils and T cells. Of type 2 diabetic pancreases with insulitis, 36% contained islets that hyperstained for HLA class I. Isolated islets from type 2 diabetic donors secreted less insulin than controls, although with preserved dynamics. Insulitis in the type 2 diabetic donors did not correlate with glucose-stimulated insulin secretion, the presence of autoantibodies, BMI or HbA1c. The current definition of insulitis cannot be used to distinguish pancreases retrieved from individuals with type 1 diabetes from those with type 2 diabetes. On the basis of our findings, we propose a revised definition of insulitis, with a positive diagnosis when ≥ 15 CD3(+) cells, not CD45(+) cells, are found in ≥ 3 islets. |
X Demographics
The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 25% |
| Canada | 4 | 25% |
| Sweden | 1 | 6% |
| Saudi Arabia | 1 | 6% |
| Germany | 1 | 6% |
| Switzerland | 1 | 6% |
| Unknown | 4 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 63% |
| Scientists | 5 | 31% |
| Practitioners (doctors, other healthcare professionals) | 1 | 6% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 20% |
| Researcher | 8 | 15% |
| Student > Bachelor | 7 | 13% |
| Other | 3 | 6% |
| Student > Doctoral Student | 3 | 6% |
| Other | 11 | 20% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 15 | 28% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Immunology and Microbiology | 6 | 11% |
| Agricultural and Biological Sciences | 4 | 7% |
| Nursing and Health Professions | 2 | 4% |
| Other | 9 | 17% |
| Unknown | 10 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2022.
All research outputs
#1,969,933
of 23,221,875 outputs
Outputs from Diabetologia
#1,048
of 5,108 outputs
Outputs of similar age
#36,638
of 314,751 outputs
Outputs of similar age from Diabetologia
#30
of 77 outputs
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