| Title |
Rheumatoid synovial fibroblasts differentiate into distinct subsets in the presence of cytokines and cartilage
|
|---|---|
| Published in |
Arthritis Research & Therapy, November 2016
|
| DOI | 10.1186/s13075-016-1156-1 |
| Pubmed ID | |
| Authors |
Adam P. Croft, Amy J. Naylor, Jennifer L. Marshall, Debbie L. Hardie, Birgit Zimmermann, Jason Turner, Guillaume Desanti, Holly Adams, Adrian I. Yemm, Ulf Müller-Ladner, Jean-Michel Dayer, Elena Neumann, Andrew Filer, Christopher D. Buckley |
| Abstract |
We investigated two distinct synovial fibroblast populations that were located preferentially in the lining or sub-lining layers and defined by their expression of either podoplanin (PDPN) or CD248, and explored their ability to undergo self-assembly and transmigration in vivo. Synovial fibroblasts (SF) were cultured in vitro and phenotypic changes following stimulation with interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β1 were examined. To examine the phenotype of SF in vivo, a severe combined immunodeficiency (SCID) human-mouse model of cartilage destruction was utilised. SF in the lining layer in rheumatoid arthritis (RA) expressed high levels of PDPN compared to the normal synovium, whereas CD248 expression was restricted to sub-lining layer cells. TNF-α or IL1 stimulation in vitro resulted in an increased expression of PDPN. In contrast, stimulation with TGF-β1 induced CD248 expression. In the SCID human-mouse model, rheumatoid SF recapitulated the expression of PDPN and CD248. Fibroblasts adjacent to cartilage expressed PDPN, and attached to, invaded, and degraded cartilage. PDPN(+) CD248(-) SF preceded the appearance of PDPN(-) CD248(+) cells in contralateral implants. We have identified two distinct SF populations identified by expression of either PDPN or CD248 which are located within different anatomical compartments of the inflamed synovial membrane. These markers discriminate between SF subsets with distinct biological properties. As PDPN-expressing cells are associated with early fibroblast migration and cartilage erosion in vivo, we propose that PDPN-expressing cells may be an attractive therapeutic target in RA. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 14% |
| Pakistan | 1 | 14% |
| United States | 1 | 14% |
| Unknown | 4 | 57% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 43% |
| Science communicators (journalists, bloggers, editors) | 2 | 29% |
| Practitioners (doctors, other healthcare professionals) | 1 | 14% |
| Members of the public | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 122 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| China | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 119 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 16% |
| Researcher | 16 | 13% |
| Student > Bachelor | 13 | 11% |
| Student > Master | 13 | 11% |
| Student > Postgraduate | 5 | 4% |
| Other | 14 | 11% |
| Unknown | 42 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 18% |
| Biochemistry, Genetics and Molecular Biology | 18 | 15% |
| Immunology and Microbiology | 17 | 14% |
| Agricultural and Biological Sciences | 14 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
| Other | 5 | 4% |
| Unknown | 44 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2021.
All research outputs
#1,070,085
of 25,373,627 outputs
Outputs from Arthritis Research & Therapy
#91
of 3,381 outputs
Outputs of similar age
#21,431
of 415,777 outputs
Outputs of similar age from Arthritis Research & Therapy
#2
of 56 outputs
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So far Altmetric has tracked 3,381 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.2. This one has done particularly well, scoring higher than 97% of its peers.
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