| Title |
Prevalence and characterisation of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in healthy volunteers in Tunisia
|
|---|---|
| Published in |
European Journal of Clinical Microbiology & Infectious Diseases, November 2011
|
| DOI | 10.1007/s10096-011-1471-z |
| Pubmed ID | |
| Authors |
R. Ben Sallem, K. Ben Slama, V. Estepa, A. Jouini, H. Gharsa, N. Klibi, Y. Sáenz, F. Ruiz-Larrea, A. Boudabous, C. Torres |
| Abstract |
The objective of this investigation was to analyse the carriage rate of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in faecal samples of healthy humans in Tunisia and to characterise the recovered isolates. One hundred and fifty samples were inoculated on MacConkey agar plates supplemented with cefotaxime (2 μg/ml) for ESBL-positive E. coli recovery. The characterisation of ESBL genes and their genetic environments, detection of associated resistance genes, multilocus sequence typing (MLST) and phylogroup typing were performed by polymerase chain reaction (PCR) and sequencing. The presence and characterisation of integrons and virulence factors were studied by PCR and sequencing. ESBL-positive E. coli isolates were detected in 11 of 150 faecal samples (7.3%) and one isolate/sample was further characterised. These isolates contained the blaCTX-M-1 (ten isolates) and blaTEM-52c genes (one isolate). The ISEcp1 (truncated by IS10 in four strains) and orf477 sequences were found upstream and downstream, respectively, of all bla (CTX-M-1) genes. Seven different sequence types (STs) and three phylogroups were identified among CTX-M-1-producing isolates [ST/phylogroup (number of isolates)]: ST58/B1 (3), ST57/D (2), ST165/A (1), ST155/B1 (1), ST10/A (1), ST398/A (1) and ST48/B1 (1). The TEM-52-producing isolate was typed as ST219 and phylogroup B2. Six ESBL isolates contained class 1 integrons with the gene cassettes dfrA17-aadA5 (five isolates) and dfrA1-aadA1 (one). Healthy humans in the studied country could be a reservoir of CTX-M-1-producing E. coli. |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 1% |
| Netherlands | 1 | 1% |
| Ethiopia | 1 | 1% |
| France | 1 | 1% |
| Pakistan | 1 | 1% |
| Estonia | 1 | 1% |
| Unknown | 85 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 19% |
| Student > Ph. D. Student | 15 | 16% |
| Student > Master | 12 | 13% |
| Student > Doctoral Student | 7 | 8% |
| Student > Postgraduate | 7 | 8% |
| Other | 17 | 19% |
| Unknown | 16 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 21 | 23% |
| Agricultural and Biological Sciences | 18 | 20% |
| Immunology and Microbiology | 13 | 14% |
| Biochemistry, Genetics and Molecular Biology | 9 | 10% |
| Veterinary Science and Veterinary Medicine | 2 | 2% |
| Other | 2 | 2% |
| Unknown | 26 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2013.
All research outputs
#20,190,878
of 22,707,247 outputs
Outputs from European Journal of Clinical Microbiology & Infectious Diseases
#2,402
of 2,767 outputs
Outputs of similar age
#131,077
of 142,974 outputs
Outputs of similar age from European Journal of Clinical Microbiology & Infectious Diseases
#23
of 27 outputs
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