| Title |
Clathrin adaptor CALM/PICALM is associated with neurofibrillary tangles and is cleaved in Alzheimer’s brains
|
|---|---|
| Published in |
Acta Neuropathologica, April 2013
|
| DOI | 10.1007/s00401-013-1111-z |
| Pubmed ID | |
| Authors |
Kunie Ando, Jean-Pierre Brion, Virginie Stygelbout, Valérie Suain, Michèle Authelet, Robert Dedecker, Anaïs Chanut, Pascale Lacor, Jérémie Lavaur, Véronique Sazdovitch, Ekaterina Rogaeva, Marie-Claude Potier, Charles Duyckaerts |
| Abstract |
PICALM, a clathrin adaptor protein, plays important roles in clathrin-mediated endocytosis in all cell types. Recently, genome-wide association studies identified single nucleotide polymorphisms in PICALM gene as genetic risk factors for late-onset Alzheimer disease (LOAD). We analysed by western blotting with several anti-PICALM antibodies the pattern of expression of PICALM in human brain extracts. We found that PICALM was abnormally cleaved in AD samples and that the level of the uncleaved 65-75 kDa full-length PICALM species was significantly decreased in AD brains. Cleavage of human PICALM after activation of endogenous calpain or caspase was demonstrated in vitro. Immunohistochemistry revealed that PICALM was associated in situ with neurofibrillary tangles, co-localising with conformationally abnormal and hyperphosphorylated tau in LOAD, familial AD and Down syndrome cases. PHF-tau proteins co-immunoprecipitated with PICALM. PICALM was highly expressed in microglia in LOAD. These observations suggest that PICALM is associated with the development of AD tau pathology. PICALM cleavage could contribute to endocytic dysfunction in AD. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 132 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Unknown | 131 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 31 | 23% |
| Researcher | 21 | 16% |
| Student > Master | 15 | 11% |
| Student > Bachelor | 10 | 8% |
| Professor | 8 | 6% |
| Other | 21 | 16% |
| Unknown | 26 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 34 | 26% |
| Neuroscience | 21 | 16% |
| Biochemistry, Genetics and Molecular Biology | 17 | 13% |
| Medicine and Dentistry | 10 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
| Other | 11 | 8% |
| Unknown | 36 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2013.
All research outputs
#18,336,865
of 22,707,247 outputs
Outputs from Acta Neuropathologica
#2,194
of 2,360 outputs
Outputs of similar age
#132,432
of 175,235 outputs
Outputs of similar age from Acta Neuropathologica
#14
of 16 outputs
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