| Title |
Combined walking exercise and alkali therapy in patients with CKD4–5 regulates intramuscular free amino acid pools and ubiquitin E3 ligase expression
|
|---|---|
| Published in |
European Journal of Applied Physiology, April 2013
|
| DOI | 10.1007/s00421-013-2628-5 |
| Pubmed ID | |
| Authors |
Emma L. Watson, George C. Kosmadakis, Alice C. Smith, Joao L. Viana, Jeremy R. Brown, Karen Molyneux, Izabella Z. A. Pawluczyk, Michael Mulheran, Nicolette C. Bishop, Susan Shirreffs, Ronald J. Maughan, Paul J. Owen, Stephen G. John, Christopher W. McIntyre, John Feehally, Alan Bevington |
| Abstract |
Muscle-wasting in chronic kidney disease (CKD) arises from several factors including sedentary behaviour and metabolic acidosis. Exercise is potentially beneficial but might worsen acidosis through exercise-induced lactic acidosis. We studied the chronic effects of exercise in CKD stage 4-5 patients (brisk walking, 30 min, 5 times/week), and non-exercising controls; each group receiving standard oral bicarbonate (STD), or additional bicarbonate (XS) (Total n = 26; Exercising + STD n = 9; Exercising +XS n = 6; Control + STD n = 8; Control + XS n = 3). Blood and vastus lateralis biopsies were drawn at baseline and 6 months. The rise in blood lactate in submaximal treadmill tests was suppressed in the Exercising + XS group. After 6 months, intramuscular free amino acids (including the branched chain amino acids) in the Exercising + STD group showed a striking chronic depletion. This did not occur in the Exercising + XS group. The effect in Exercising + XS patients was accompanied by reduced transcription of ubiquitin E3-ligase MuRF1 which activates proteolysis via the ubiquitin-proteasome pathway. Other anabolic indicators (Akt activation and suppression of the 14 kDa actin catabolic marker) were unaffected in Exercising + XS patients. Possibly because of this, overall suppression of myofibrillar proteolysis (3-methylhistidine output) was not observed. It is suggested that alkali effects in exercisers arose by countering exercise-induced acidosis. Whether further anabolic effects are attainable on combining alkali with enhanced exercise (e.g. resistance exercise) merits further investigation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 33% |
| Portugal | 1 | 33% |
| United Kingdom | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 91 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 15% |
| Student > Master | 11 | 12% |
| Student > Ph. D. Student | 10 | 11% |
| Student > Bachelor | 9 | 10% |
| Student > Postgraduate | 6 | 7% |
| Other | 12 | 13% |
| Unknown | 29 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 23 | 25% |
| Nursing and Health Professions | 11 | 12% |
| Biochemistry, Genetics and Molecular Biology | 8 | 9% |
| Sports and Recreations | 6 | 7% |
| Agricultural and Biological Sciences | 4 | 4% |
| Other | 8 | 9% |
| Unknown | 31 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 September 2023.
All research outputs
#15,740,505
of 25,374,917 outputs
Outputs from European Journal of Applied Physiology
#2,985
of 4,345 outputs
Outputs of similar age
#120,876
of 209,588 outputs
Outputs of similar age from European Journal of Applied Physiology
#19
of 27 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,345 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.6. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,588 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.