| Title |
Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat
|
|---|---|
| Published in |
BMC Plant Biology, October 2016
|
| DOI | 10.1186/s12870-016-0916-z |
| Pubmed ID | |
| Authors |
Shengnan Zhai, Genying Li, Youwei Sun, Jianmin Song, Jihu Li, Guoqi Song, Yulian Li, Hongqing Ling, Zhonghu He, Xianchun Xia |
| Abstract |
Phytoene synthase 1 (PSY1) is the most important regulatory enzyme in carotenoid biosynthesis, whereas its function is hardly known in common wheat. The aims of the present study were to investigate Psy1 function and genetic regulation using reverse genetics approaches. Transcript levels of Psy1 in RNAi transgenic lines were decreased by 54-76 % and yellow pigment content (YPC) was reduced by 26-35 % compared with controls, confirming the impact of Psy1 on carotenoid accumulation. A series of candidate genes involved in secondary metabolic pathways and core metabolic processes responded to Psy1 down-regulation. The aspartate rich domain (DXXXD) was important for PSY1 function, and conserved nucleotides adjacent to the domain influenced YPC by regulating gene expression, enzyme activity or alternative splicing. Compensatory responses analysis indicated that three Psy1 homoeologs may be coordinately regulated under normal conditions, but separately regulated under stress. The period 14 days post anthesis (DPA) was found to be a key regulation node during grain development. The findings define key aspects of flour color regulation in wheat and facilitate the genetic improvement of wheat quality targeting color/nutritional specifications required for specific end products. |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 2% |
| Unknown | 44 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 24% |
| Researcher | 7 | 16% |
| Student > Ph. D. Student | 5 | 11% |
| Other | 3 | 7% |
| Student > Bachelor | 2 | 4% |
| Other | 5 | 11% |
| Unknown | 12 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 51% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Arts and Humanities | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Unknown | 15 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2016.
All research outputs
#4,196,076
of 22,903,988 outputs
Outputs from BMC Plant Biology
#300
of 3,269 outputs
Outputs of similar age
#70,506
of 316,330 outputs
Outputs of similar age from BMC Plant Biology
#6
of 42 outputs
Altmetric has tracked 22,903,988 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,269 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,330 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.