| Title |
Proteomic Analysis of Differential Proteins Related to Anti-nociceptive Effect of Electroacupuncture in the Hypothalamus Following Neuropathic Pain in Rats
|
|---|---|
| Published in |
Neurochemical Research, April 2013
|
| DOI | 10.1007/s11064-013-1047-7 |
| Pubmed ID | |
| Authors |
Yonghui Gao, Shuping Chen, Qiuling Xu, Kan Yu, Junying Wang, Lina Qiao, Fanying Meng, Junling Liu |
| Abstract |
Increasing evidence has been accumulated for the effectiveness of acupuncture therapy in relieving pain. However, there are limited data on regulation of protein expression after electroacupuncture (EA) intervention. Thus, the present study is designed to determine changes in protein expression following EA stimulation in rats with sciatic nerve chronic constrictive injury (CCI) induced neuropathic pain. Sixty Wistar rats were equally randomized into normal control group, CCI group, and CCI with EA stimulation (EA) group. The CCI model was established by ligature of the left sciatic nerve. EA stimulation was applied at Zusanli (ST36) and Yanglingquan (GB34) in the EA group. Differentially expressed hypothalamic proteins in the three groups were identified by 2-D gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The functional clustering and pathway of the identified proteins were analyzed by Mascot software. Results showed that, after CCI, the thermal pain threshold of the affected hind footpad was decreased and was reversed gradually by 12 sessions of EA treatment. Following EA intervention, there were 17 hypothalamic proteins identified with significant changes in the expression (>twofold). Three gene-ontologies (oxidoreductase activity, oxidation reduction, and protein binding) were enriched, while there was a significant regulation of glycolysis/gluconeogenesis/hexose metabolism pathway. These data demonstrate that EA intervention can attenuate pain via regulation of expression of multiple proteins in the hypothalamus. Further, hypothalamic glucose metabolism may be important in supporting energy and neurotransmitter homeostasis in the effects of EA intervention. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 31% |
| Researcher | 4 | 14% |
| Student > Doctoral Student | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Unspecified | 2 | 7% |
| Other | 6 | 21% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 9 | 31% |
| Agricultural and Biological Sciences | 5 | 17% |
| Nursing and Health Professions | 3 | 10% |
| Unspecified | 2 | 7% |
| Neuroscience | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 4 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2013.
All research outputs
#18,337,420
of 22,708,120 outputs
Outputs from Neurochemical Research
#1,482
of 2,089 outputs
Outputs of similar age
#147,138
of 195,119 outputs
Outputs of similar age from Neurochemical Research
#18
of 25 outputs
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