| Title |
Metabolic and morphological changes of an oil accumulating trebouxiophycean alga in nitrogen-deficient conditions
|
|---|---|
| Published in |
Metabolomics, September 2012
|
| DOI | 10.1007/s11306-012-0463-z |
| Pubmed ID | |
| Authors |
Takuro Ito, Miho Tanaka, Haruka Shinkawa, Takashi Nakada, Yoshitaka Ano, Norihide Kurano, Tomoyoshi Soga, Masaru Tomita |
| Abstract |
Oil-rich algae have promising potential for a next-generation biofuel feedstock. Pseudochoricystis ellipsoidea MBIC 11204, a novel unicellular green algal strain, accumulates a large amount of oil (lipids) in nitrogen-deficient (-N) conditions. Although the oil bodies are easily visualized by lipophilic staining in the cells, little is known about how oil bodies are metabolically synthesized. Clarifying the metabolic profiles in -N conditions is important to understand the physiological mechanisms of lipid accumulations and will be useful to optimize culture conditions efficiently produce industrial oil. Metabolome and lipidome profiles were obtained, respectively, using capillary electrophoresis- and liquid chromatography-mass spectrometry from P. ellipsoidea in both nitrogen-rich (+N; rapid growth) and -N conditions. Relative quantities of more than 300 metabolites were systematically compared between these two conditions. Amino acids in nitrogen assimilation and N-transporting metabolisms were decreased to 1/20 the amount, or less, in -N conditions. In lipid metabolism, the quantities of neutral lipids increased greatly in -N conditions; however, quantities of nearly all the other lipids either decreased or only changed slightly. The morphological changes in +N and -N conditions were also provided by microscopy, and we discuss their relationship to the metabolic changes. This is the first approach to understand the novel algal strain's metabolism using a combination of wide-scale metabolome analysis and morphological analysis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 134 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| Malaysia | 1 | <1% |
| India | 1 | <1% |
| Japan | 1 | <1% |
| Unknown | 129 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 20% |
| Researcher | 22 | 16% |
| Student > Master | 21 | 16% |
| Student > Bachelor | 13 | 10% |
| Student > Doctoral Student | 12 | 9% |
| Other | 15 | 11% |
| Unknown | 24 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 60 | 45% |
| Biochemistry, Genetics and Molecular Biology | 14 | 10% |
| Environmental Science | 8 | 6% |
| Energy | 4 | 3% |
| Chemical Engineering | 3 | 2% |
| Other | 13 | 10% |
| Unknown | 32 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2013.
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#18,337,420
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Outputs from Metabolomics
#1,071
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Outputs of similar age
#130,704
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Outputs of similar age from Metabolomics
#8
of 16 outputs
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