| Title |
Insect cells respiratory activity in bioreactor
|
|---|---|
| Published in |
Methods in Cell Science, February 2008
|
| DOI | 10.1007/s10616-007-9118-8 |
| Pubmed ID | |
| Authors |
Marilena Martins Pamboukian, Soraia Athie Calil Jorge, Mariza Gerdulo Santos, Adriana Yurie Yokomizo, Carlos Augusto Pereira, Aldo Tonso |
| Abstract |
Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Spodoptera frugiperda (Sf9) cells have been used through virus infection as host for foreign protein expression and bioinsecticide production. Transfected Drosophila melanogaster (S2) cells can be used to produce different proteins. The objective of this work is to investigate respiratory activity and oxygen transfer during the growth of different insect cells lines as Spodoptera frugiperda (Sf9), Drosophila melanogaster (S2) wild and transfected for the expression of GPV and EGFP. All experiments were performed in a well-controlled 1-L bioreactor, with SF900II serum free medium. Spodoptera frugiperda (Sf9) cells reached 10.7 x 10(6) cells/mL and maximum specific respiration rate ([Formula: see text]) of 7.3 x 10(-17) molO(2)/cell s. Drosophila melanogaster (S2) cells achieved 51.2 x 10(6) cells/mL and [Formula: see text] of 3.1 x 10(-18) molO(2)/cell s. S2AcGPV (expressing with rabies virus glycoprotein) reached 24.9 x 10(6) cells/mL and [Formula: see text] of 1.7 x 10(-17) molO(2)/cell s, while S2MtEGFP (expressing green fluorescent protein) achieved 15.5 x 10(6) cells/mL and [Formula: see text] = 1.9 x 10(-17) molO(2)/cell s. Relating to the Sf9, S2 cells reached higher maximum cell concentrations and lower specific respiration rate, which can be explained by its smaller size. These results presented useful information for scale-up and process control of insect cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 4% |
| Mexico | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 49 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 28% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Bachelor | 6 | 11% |
| Researcher | 4 | 8% |
| Other | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 9 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 30% |
| Engineering | 10 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Veterinary Science and Veterinary Medicine | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 10 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2022.
All research outputs
#8,535,684
of 25,374,917 outputs
Outputs from Methods in Cell Science
#356
of 1,026 outputs
Outputs of similar age
#33,757
of 95,095 outputs
Outputs of similar age from Methods in Cell Science
#3
of 12 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,026 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
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