| Title |
Epigenetic Signature of Early Cardiac Regulatory Genes in Native Human Adipose-Derived Stem Cells
|
|---|---|
| Published in |
Cell Biochemistry and Biophysics, April 2013
|
| DOI | 10.1007/s12013-013-9610-z |
| Pubmed ID | |
| Authors |
Alice Pasini, Francesca Bonafè, Marco Govoni, Carlo Guarnieri, Paolo G. Morselli, Hari S. Sharma, Claudio M. Caldarera, Claudio Muscari, Emanuele Giordano |
| Abstract |
Adipose-derived stem cells (ADSCs) are stromal mesenchymal stem cells isolated from lipoaspirates, and they display a broad potential to differentiate toward different lineages. The role of epigenetics in regulating the expression of their lineage-specific genes is under evaluation, however till date virtually nothing is known about the relative significance of cardiac-specific transcription factor genes in human ADSCs. The aim of this study was to investigate DNA promoter methylation and relevant histone modifications involving MEF-2C, GATA-4, and Nkx2.5 in native human ADSCs. CpG sites at the transcription start in their promoters were found unmethylated using methylation-specific PCR. Chromatin immunoprecipitation assay showed low levels of total acetylated H3 histone (acH3) and high levels of trimethylated lysine 27 in H3 histone (H3K27me3) which were associated with both GATA-4 and Nkx2.5 promoters, indicating their transcriptional repressive chromatin arrangement. On the other hand, the opposite was apparent for MEF-2C promoter. Accordingly, MEF-2C-but not GATA-4 and Nkx2.5-transcripts were evidenced in native human ADSCs. These results suggest that the chromatin arrangement of these early cardiac regulatory genes could be explored as a level of intervention to address the differentiation of human ADSCs toward the cardiac lineage. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 7% |
| Switzerland | 1 | 7% |
| Unknown | 13 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 27% |
| Student > Ph. D. Student | 4 | 27% |
| Researcher | 3 | 20% |
| Professor | 2 | 13% |
| Professor > Associate Professor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 33% |
| Agricultural and Biological Sciences | 4 | 27% |
| Engineering | 3 | 20% |
| Medicine and Dentistry | 1 | 7% |
| Arts and Humanities | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2013.
All research outputs
#14,751,991
of 22,708,120 outputs
Outputs from Cell Biochemistry and Biophysics
#335
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Outputs of similar age
#115,261
of 193,472 outputs
Outputs of similar age from Cell Biochemistry and Biophysics
#24
of 38 outputs
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