| Title |
Alteration of cell responses to PrPSc in prolonged cell culture and its effect on transmission of PrPSc to neural cells
|
|---|---|
| Published in |
Archives of Virology, November 2012
|
| DOI | 10.1007/s00705-012-1540-3 |
| Pubmed ID | |
| Authors |
Abdelazim E. Elhelaly, Yasuo Inoshima, Naotaka Ishiguro |
| Abstract |
The mechanisms and processes of the uptake, intracellular trafficking and intercellular spread of PrP(Sc) and its transfer to neural cells are not clearly defined. The involvement of immune, intestinal, mast or peripheral neural cells in this process also remains unclear. The role of these cell types in the accumulation and transfer of PrP(Sc) to neural cells was investigated following short and prolonged exposure to the Chandler and Obihiro strains of scrapie PrP(Sc) for up to 28 days. Eight cell lines of murine immune, neural, intestinal and fibroblast cell types were tested. After transient degradation phases, certain immune, intestinal and neural cells accumulated PrP(Sc) for up to 28 days postinfection. When co-cultured with N2a-3/EGFP neuroblastoma cells for 4 days followed by several passages, the immune, intestinal and the neural cell lines were able to transfer infection to neural cells. Our results suggest that some of these cell types may have a role in PrP(Sc) accumulation and intercellular spread of PrP(Sc) infection to neural cells in vivo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 30% |
| Professor | 2 | 20% |
| Other | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 50% |
| Veterinary Science and Veterinary Medicine | 2 | 20% |
| Nursing and Health Professions | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Immunology and Microbiology | 1 | 10% |
| Other | 0 | 0% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2013.
All research outputs
#18,338,033
of 22,709,015 outputs
Outputs from Archives of Virology
#3,004
of 4,132 outputs
Outputs of similar age
#213,947
of 275,782 outputs
Outputs of similar age from Archives of Virology
#19
of 27 outputs
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