| Title |
Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, February 2011
|
| DOI | 10.1007/s12015-011-9239-5 |
| Pubmed ID | |
| Authors |
Kristina Nagy, Hoon-Ki Sung, Puzheng Zhang, Simon Laflamme, Patrick Vincent, Siamak Agha-Mohammadi, Knut Woltjen, Claudio Monetti, Iacovos Prodromos Michael, Lawrence Charles Smith, Andras Nagy |
| Abstract |
The domesticated horse represents substantial value for the related sports and recreational fields, and holds enormous potential as a model for a range of medical conditions commonly found in humans. Most notable of these are injuries to muscles, tendons, ligaments and joints. Induced pluripotent stem (iPS) cells have sparked tremendous hopes for future regenerative therapies of conditions that today are not possible to cure. Equine iPS (EiPS) cells, in addition to bringing promises to the veterinary field, open up the opportunity to utilize horses for the validation of stem cell based therapies before moving into the human clinical setting. In this study, we report the generation of iPS cells from equine fibroblasts using a piggyBac (PB) transposon-based method to deliver transgenes containing the reprogramming factors Oct4, Sox2, Klf4 and c-Myc, expressed in a temporally regulated fashion. The established iPS cell lines express hallmark pluripotency markers, display a stable karyotype even during long-term culture, and readily form complex teratomas containing all three embryonic germ layer derived tissues upon in vivo grafting into immunocompromised mice. Our EiPS cell lines hold the promise to enable the development of a whole new range of stem cell-based regenerative therapies in veterinary medicine, as well as aid the development of preclinical models for human applications. EiPS cell could also potentially be used to revive recently extinct or currently threatened equine species. |
Mendeley readers
The data shown below were compiled from readership statistics for 190 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 4 | 2% |
| Brazil | 4 | 2% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Iran, Islamic Republic of | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 178 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 41 | 22% |
| Researcher | 36 | 19% |
| Student > Master | 26 | 14% |
| Student > Bachelor | 11 | 6% |
| Student > Postgraduate | 11 | 6% |
| Other | 31 | 16% |
| Unknown | 34 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 76 | 40% |
| Biochemistry, Genetics and Molecular Biology | 25 | 13% |
| Medicine and Dentistry | 21 | 11% |
| Veterinary Science and Veterinary Medicine | 18 | 9% |
| Immunology and Microbiology | 3 | 2% |
| Other | 12 | 6% |
| Unknown | 35 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2023.
All research outputs
#2,483,399
of 25,373,627 outputs
Outputs from Stem Cell Reviews and Reports
#46
of 1,035 outputs
Outputs of similar age
#10,378
of 117,704 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#1
of 18 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,035 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 117,704 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.