| Title |
Antibiotic resistance and clonal diversity of invasive Staphylococcus aureus in the rural Ashanti Region, Ghana
|
|---|---|
| Published in |
BMC Infectious Diseases, November 2016
|
| DOI | 10.1186/s12879-016-2048-3 |
| Pubmed ID | |
| Authors |
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G. Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May |
| Abstract |
Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S. aureus isolates was carried out by the VITEK 2 system. Multiplex polymerase chain reaction (PCR) was used to detect S. aureus-specific nuc gene, Panton-Valentine leukocidin (PVL), and methicillin-resistant S. aureus (MRSA)-specific mecA and mecC genes. The population structure of S. aureus was assessed by spa typing. In total, 9,834 blood samples were cultured, out of which 0.6% (n = 56) were positive for S. aureus. Multidrug resistance (MDR) was detected in 35.7% (n = 20) of the S. aureus strains, of which one was a MRSA. The highest rate of antibiotic resistance was seen for commonly available antibiotics, including penicillin (n = 55; 98.2%), tetracycline (n = 32; 57.1%) and trimethoprim/sulfamethoxazole (n = 26; 46.4%). Of all S. aureus strains, 75.0% (n = 42) carried the PVL-encoding genes. We found 25 different spa types with t355 (n = 11; 19.6%), t314 (n = 8; 14.3%), t084 (n = 8; 14.3%) and t311 (n = 5; 8.9%) being predominant. The study exhibited an alarmingly large level of antibiotic resistance to locally available antibiotics. The frequency of genetically diverse and PVL-positive methicillin-sensitive S. aureus (MSSA) was high and could represent a reservoir for the emergence of virulent PVL-positive MRSA clones. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Venezuela, Bolivarian Republic of | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 1% |
| Unknown | 98 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 19 | 19% |
| Researcher | 12 | 12% |
| Student > Bachelor | 10 | 10% |
| Student > Postgraduate | 7 | 7% |
| Student > Ph. D. Student | 4 | 4% |
| Other | 13 | 13% |
| Unknown | 34 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 19 | 19% |
| Biochemistry, Genetics and Molecular Biology | 15 | 15% |
| Agricultural and Biological Sciences | 9 | 9% |
| Immunology and Microbiology | 7 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
| Other | 6 | 6% |
| Unknown | 39 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 December 2016.
All research outputs
#18,483,671
of 22,903,988 outputs
Outputs from BMC Infectious Diseases
#5,620
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#304,685
of 416,538 outputs
Outputs of similar age from BMC Infectious Diseases
#153
of 212 outputs
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