| Title |
Proteomic profile of maternal-aged blastocoel fluid suggests a novel role for ubiquitin system in blastocyst quality
|
|---|---|
| Published in |
Journal of Assisted Reproduction and Genetics, December 2016
|
| DOI | 10.1007/s10815-016-0842-x |
| Pubmed ID | |
| Authors |
Gabriella Tedeschi, Elena Albani, Elena Monica Borroni, Valentina Parini, Anna Maria Brucculeri, Elisa Maffioli, Armando Negri, Simona Nonnis, Mauro Maccarrone, Paolo Emanuele Levi-Setti |
| Abstract |
The etiology of maternal aging, a common cause of female factor infertility and a rate-limiting step in vitro fertilization (IVF) success, remains still unclear. Proteomic changes responsible for the impaired successful pregnancy outcome after IVF with aged blastocysts have not been yet evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to enlight differences at the protein level in blastocoel fluid of aged and younger woman. Protein composition of human blastocoel fluid isolated by micromanipulation from 46 blastocysts of women aged <37 years (group A) and 29 of women aged ≥37 years (group B) have been identified by a shotgun proteomic approach based on high-resolution nano-liquid chromatography electrospray-ionization-tandem mass spectrometry (nLC-ESI-MS/MS) using label free for the relative quantification of their expression levels. The proteomic analysis leads to the identification and quantification of 148 proteins; 132 and 116 proteins were identified in groups A and B, respectively. Interestingly, the identified proteins are mainly involved in processes aimed at fine tuning embryo implantation and development. Among the 100 proteins commonly expressed in both groups, 17 proteins are upregulated and 44 downregulated in group B compared to group A. Overall, the analysis identified 33 proteins, which were increased or present only in B while 76 were decreased in B or present only in A. Data revealed that maternal aging mainly affects blastocyst survival and implantation through unbalancing the equilibrium of the ubiquitin system known to play a crucial role in fine-tuning several aspects required to ensure successful pregnancy outcome. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 22% |
| Researcher | 5 | 14% |
| Lecturer | 4 | 11% |
| Student > Postgraduate | 3 | 8% |
| Unspecified | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 10 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 10 | 27% |
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Unspecified | 2 | 5% |
| Agricultural and Biological Sciences | 2 | 5% |
| Arts and Humanities | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 15 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2016.
All research outputs
#21,608,038
of 24,119,703 outputs
Outputs from Journal of Assisted Reproduction and Genetics
#1,433
of 1,697 outputs
Outputs of similar age
#362,165
of 427,346 outputs
Outputs of similar age from Journal of Assisted Reproduction and Genetics
#17
of 18 outputs
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