Title |
Molecular analysis of transcription start sites of separate copies of the suffix short retroelement in genome of Drosophila
|
---|---|
Published in |
Doklady Biochemistry and Biophysics, May 2013
|
DOI | 10.1134/s1607672913020063 |
Pubmed ID | |
Authors |
O. S. Zenina, D. M. Fedoseeva, O. V. Kretova, N. A. Tchurikov |
Abstract |
In order to study TSS in the suffix element, we used total RNA isolated from ovaries of Drosophila melanogaster. Using a 5'-RACE System (Invitrogen) and 454 sequencing, we found the full-length suffix sense transcripts. However, most 5'-RACE reads (>70%) correspond to 5'-truncated transcripts lacking the first 33-39 nucleotides. The data may indicate that these RNAs are products of the RNAi-related silencing mech-anism producing small RNAs that are larger than piRNAs. The full-length suffix transcripts could arise either from the F element or from active separate copies of suffix. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Unknown | 1 | 33% |