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Sequencing platform and library preparation choices impact viral metagenomes

Overview of attention for article published in BMC Genomics, May 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

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20 X users
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1 patent
facebook
1 Facebook page
googleplus
1 Google+ user

Citations

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88 Dimensions

Readers on

mendeley
265 Mendeley
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4 CiteULike
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Title
Sequencing platform and library preparation choices impact viral metagenomes
Published in
BMC Genomics, May 2013
DOI 10.1186/1471-2164-14-320
Pubmed ID
Authors

Sergei A Solonenko, J César Ignacio-Espinoza, Adriana Alberti, Corinne Cruaud, Steven Hallam, Kostas Konstantinidis, Gene Tyson, Patrick Wincker, Matthew B Sullivan

Abstract

Microbes drive the biogeochemistry that fuels the planet. Microbial viruses modulate their hosts directly through mortality and horizontal gene transfer, and indirectly by re-programming host metabolisms during infection. However, our ability to study these virus-host interactions is limited by methods that are low-throughput and heavily reliant upon the subset of organisms that are in culture. One way forward are culture-independent metagenomic approaches, but these novel methods are rarely rigorously tested, especially for studies of environmental viruses, air microbiomes, extreme environment microbiology and other areas with constrained sample amounts. Here we perform replicated experiments to evaluate Roche 454, Illumina HiSeq, and Ion Torrent PGM sequencing and library preparation protocols on virus metagenomes generated from as little as 10 pg of DNA.

X Demographics

X Demographics

The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 265 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 10 4%
Brazil 4 2%
United Kingdom 3 1%
Spain 3 1%
Chile 1 <1%
Austria 1 <1%
Australia 1 <1%
France 1 <1%
Sweden 1 <1%
Other 7 3%
Unknown 233 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 71 27%
Student > Ph. D. Student 63 24%
Student > Master 31 12%
Professor > Associate Professor 17 6%
Student > Postgraduate 13 5%
Other 46 17%
Unknown 24 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 136 51%
Biochemistry, Genetics and Molecular Biology 38 14%
Environmental Science 14 5%
Immunology and Microbiology 12 5%
Computer Science 9 3%
Other 22 8%
Unknown 34 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2023.
All research outputs
#2,439,844
of 25,711,518 outputs
Outputs from BMC Genomics
#628
of 11,306 outputs
Outputs of similar age
#19,946
of 206,313 outputs
Outputs of similar age from BMC Genomics
#15
of 173 outputs
Altmetric has tracked 25,711,518 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,306 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 206,313 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 173 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.