Title |
Sequencing platform and library preparation choices impact viral metagenomes
|
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Published in |
BMC Genomics, May 2013
|
DOI | 10.1186/1471-2164-14-320 |
Pubmed ID | |
Authors |
Sergei A Solonenko, J César Ignacio-Espinoza, Adriana Alberti, Corinne Cruaud, Steven Hallam, Kostas Konstantinidis, Gene Tyson, Patrick Wincker, Matthew B Sullivan |
Abstract |
Microbes drive the biogeochemistry that fuels the planet. Microbial viruses modulate their hosts directly through mortality and horizontal gene transfer, and indirectly by re-programming host metabolisms during infection. However, our ability to study these virus-host interactions is limited by methods that are low-throughput and heavily reliant upon the subset of organisms that are in culture. One way forward are culture-independent metagenomic approaches, but these novel methods are rarely rigorously tested, especially for studies of environmental viruses, air microbiomes, extreme environment microbiology and other areas with constrained sample amounts. Here we perform replicated experiments to evaluate Roche 454, Illumina HiSeq, and Ion Torrent PGM sequencing and library preparation protocols on virus metagenomes generated from as little as 10 pg of DNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | 35% |
Cameroon | 1 | 5% |
Norway | 1 | 5% |
Brazil | 1 | 5% |
Canada | 1 | 5% |
Hong Kong | 1 | 5% |
Chile | 1 | 5% |
Germany | 1 | 5% |
North Macedonia | 1 | 5% |
Other | 3 | 15% |
Unknown | 2 | 10% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 10 | 50% |
Members of the public | 7 | 35% |
Science communicators (journalists, bloggers, editors) | 2 | 10% |
Practitioners (doctors, other healthcare professionals) | 1 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 10 | 4% |
Brazil | 4 | 2% |
United Kingdom | 3 | 1% |
Spain | 3 | 1% |
Chile | 1 | <1% |
Austria | 1 | <1% |
Australia | 1 | <1% |
France | 1 | <1% |
Sweden | 1 | <1% |
Other | 7 | 3% |
Unknown | 233 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 71 | 27% |
Student > Ph. D. Student | 63 | 24% |
Student > Master | 31 | 12% |
Professor > Associate Professor | 17 | 6% |
Student > Postgraduate | 13 | 5% |
Other | 46 | 17% |
Unknown | 24 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 136 | 51% |
Biochemistry, Genetics and Molecular Biology | 38 | 14% |
Environmental Science | 14 | 5% |
Immunology and Microbiology | 12 | 5% |
Computer Science | 9 | 3% |
Other | 22 | 8% |
Unknown | 34 | 13% |