| Title |
Effects of Bacillus cereus Endospores on Free-Living Protist Growth
|
|---|---|
| Published in |
Microbial Ecology, December 2016
|
| DOI | 10.1007/s00248-016-0905-7 |
| Pubmed ID | |
| Authors |
Susana S. Santos, Niels Bohse Hendriksen, Hans Henrik Jakobsen, Anne Winding |
| Abstract |
We studied the predator-prey interactions between heterotrophic protists and endospores of Bacillus cereus group bacteria, in order to gain insight on survival and dispersal of B. cereus endospores in the environment. It has been hypothesised that the spore stage protects against digestion by predating protists. Therefore, experiments were carried out to investigate the impact of B. cereus endospores and vegetative cells, as the only food source, on individual amoeboid, flagellated and ciliated protists. The presence of fluorescent-labelled intracellular bacteria confirmed that B. cereus endospores as well as vegetative cells were ingested by protists and appeared intact in the food vacuoles when observed by epifluorescence microscopy. Furthermore, protist growth and bacterial predation were followed by qPCR. Protists were able to grow on vegetative cells as well as endospores of B. cereus, despite the lower cell division rates observed for some protists when feeding on bacterial endospores. Survival and proliferation of ingested bacteria inside protists cells was also observed. Finally, B. cereus spore germination and growth was observed within all protists with higher abundance in the amoeboid protist after antibiotic treatment of the protist surface. These observations support that protists can act as a potential breeding ground for B. cereus endospores. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 33% |
| Members of the public | 1 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 4 | 17% |
| Other | 2 | 8% |
| Student > Postgraduate | 2 | 8% |
| Student > Bachelor | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 7 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Environmental Science | 2 | 8% |
| Earth and Planetary Sciences | 2 | 8% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 9 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2016.
All research outputs
#15,402,296
of 22,912,409 outputs
Outputs from Microbial Ecology
#1,471
of 2,061 outputs
Outputs of similar age
#253,857
of 419,655 outputs
Outputs of similar age from Microbial Ecology
#37
of 48 outputs
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