| Title |
Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes
|
|---|---|
| Published in |
BMC Molecular and Cell Biology, December 2016
|
| DOI | 10.1186/s12867-016-0077-5 |
| Pubmed ID | |
| Authors |
Nicholas W. Ashton, Dorothy Loo, Nicolas Paquet, Kenneth J. O’Byrne, Derek J. Richard |
| Abstract |
Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one such protein, with described roles in genome stability maintenance and transcriptional regulation. As yet, however, the mechanisms through which hSSB1 functions and the binding partners with which it interacts remain poorly understood. In this work, hSSB1 was immunoprecipitated from cell lysate samples that had been enriched for non-soluble nuclear proteins and those associating with hSSB1 identified by mass spectrometry. In doing so, 334 potential hSSB1-associating proteins were identified, with known roles in a range of distinct biological processes. Unexpectedly, whilst hSSB1 has largely been studied in a genome stability context, few other DNA repair or replication proteins were detected. By contrast, a large number of proteins were identified with roles in mRNA metabolism, reflecting a currently emerging area of hSSB1 study. In addition, numerous proteins were detected that comprise various chromatin-remodelling complexes. These findings provide new insight into the binding partners of hSSB1 and will likely function as a platform for future research. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Venezuela, Bolivarian Republic of | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 33% |
| Student > Ph. D. Student | 5 | 24% |
| Student > Bachelor | 2 | 10% |
| Student > Master | 1 | 5% |
| Unknown | 6 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 48% |
| Agricultural and Biological Sciences | 2 | 10% |
| Medicine and Dentistry | 2 | 10% |
| Economics, Econometrics and Finance | 1 | 5% |
| Neuroscience | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 5 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2017.
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#19,944,091
of 25,373,627 outputs
Outputs from BMC Molecular and Cell Biology
#896
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Outputs of similar age
#301,740
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Outputs of similar age from BMC Molecular and Cell Biology
#6
of 8 outputs
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