| Title |
Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2011
|
| DOI | 10.1007/s00253-011-3102-0 |
| Pubmed ID | |
| Authors |
Charles F. Budde, Sebastian L. Riedel, Florian Hübner, Stefan Risch, Milan K. Popović, ChoKyun Rha, Anthony J. Sinskey |
| Abstract |
Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by bacteria for carbon and energy storage that also have commercial potential as bioplastics. One promising class of carbon feedstocks for industrial PHA production is plant oils, due to the high carbon content of these compounds. The bacterium Ralstonia eutropha accumulates high levels of PHA and can effectively utilize plant oil. Growth experiments that include plant oil, however, are difficult to conduct in a quantitative and reproducible manner due to the heterogeneity of the two-phase medium. In order to overcome this obstacle, a new culture method was developed in which palm oil was emulsified in growth medium using the glycoprotein gum arabic as the emulsifying agent. Gum arabic did not influence R. eutropha growth and could not be used as a nutrient source by the bacteria. R. eutropha was grown in the emulsified oil medium and PHA production was measured over time. Additionally, an extraction method was developed to monitor oil consumption. The new method described in this study allows quantitative, reproducible R. eutropha experiments to be performed with plant oils. The method may also prove useful for studying growth of different bacteria on plant oils and other hydrophobic carbon sources. |
Mendeley readers
The data shown below were compiled from readership statistics for 231 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 3 | 1% |
| Indonesia | 1 | <1% |
| United States | 1 | <1% |
| Austria | 1 | <1% |
| Unknown | 225 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 47 | 20% |
| Student > Bachelor | 34 | 15% |
| Student > Master | 26 | 11% |
| Researcher | 24 | 10% |
| Student > Doctoral Student | 14 | 6% |
| Other | 28 | 12% |
| Unknown | 58 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 58 | 25% |
| Biochemistry, Genetics and Molecular Biology | 36 | 16% |
| Engineering | 21 | 9% |
| Chemical Engineering | 15 | 6% |
| Environmental Science | 9 | 4% |
| Other | 22 | 10% |
| Unknown | 70 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2021.
All research outputs
#1,673,892
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#107
of 8,034 outputs
Outputs of similar age
#9,286
of 190,794 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#3
of 54 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 190,794 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.