| Title |
Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, July 2010
|
| DOI | 10.1007/s12015-010-9165-y |
| Pubmed ID | |
| Authors |
Ingrida Majore, Pierre Moretti, Frank Stahl, Ralf Hass, Cornelia Kasper |
| Abstract |
Up to 2.8 × 10(7) fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical cord (UC) using a simple and highly reproducible explant culture approach. Cells derived from whole UC, similar to cells collected from separate compartments of UC, display a distinct chondrogenic and adipogenic potential. Therefore they are potential candidates for cartilage and adipose tissue engineering. Cell differentiation along the osteogenic pathway is, however, less efficient, even after the addition of 1.25-dihydroxyvitamin D3, a potent osteoinductive substance. Isolated cells are highly proliferative, tolerate cryopreservation with an average survival rate of about 75% and after thawing can be propagated further, at least over 20 population doublings before their proliferative activity begins to decline. More importantly, they synthesize numerous trophic factors including neurotrophins and factors which facilitate angiogenesis and hematopoiesis. In conclusion, cells isolated from whole UC satisfies all requirements essential for the generation of stem cell banks containing permanently available cell material for applications in the field of regenerative medicine. Nevertheless, further studies are needed to improve and adjust the methods which are already employed for adult MSC expansion and differentiation to specific properties and requirements of the primitive stem cells collected from UC. So, our data verify that the choice of individual parameters for cell propagation, such as duration of cell expansion and cell seeding density, has a substantial impact on the quality of UC-derived cell populations. |
Mendeley readers
The data shown below were compiled from readership statistics for 162 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 4 | 2% |
| United Kingdom | 2 | 1% |
| Portugal | 1 | <1% |
| Switzerland | 1 | <1% |
| Brazil | 1 | <1% |
| India | 1 | <1% |
| Singapore | 1 | <1% |
| Mexico | 1 | <1% |
| Argentina | 1 | <1% |
| Other | 3 | 2% |
| Unknown | 146 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 32 | 20% |
| Researcher | 31 | 19% |
| Student > Master | 19 | 12% |
| Student > Bachelor | 13 | 8% |
| Student > Doctoral Student | 12 | 7% |
| Other | 28 | 17% |
| Unknown | 27 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 53 | 33% |
| Medicine and Dentistry | 23 | 14% |
| Biochemistry, Genetics and Molecular Biology | 21 | 13% |
| Engineering | 6 | 4% |
| Neuroscience | 4 | 2% |
| Other | 18 | 11% |
| Unknown | 37 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2023.
All research outputs
#2,376,145
of 25,374,647 outputs
Outputs from Stem Cell Reviews and Reports
#40
of 1,036 outputs
Outputs of similar age
#8,687
of 104,125 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#1
of 17 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,036 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 104,125 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.