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Bioreactor for Blood Product Production

Overview of attention for article published in Cell Transplantation, June 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

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blogs
1 blog
twitter
2 X users
patent
1 patent

Citations

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7 Dimensions

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36 Mendeley
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Title
Bioreactor for Blood Product Production
Published in
Cell Transplantation, June 2012
DOI 10.3727/096368911x627363
Pubmed ID
Authors

Michael R. Doran, Ian Alexander Aird, Flavia Marturana, Nicholas Timmins, Kerry Atkinson, Lars K. Nielsen

Abstract

The feasibility of ex vivo blood production is limited by both biological and engineering challenges. From an engineering perspective, these challenges include the significant volumes required to generate even a single unit of a blood product, as well as the correspondingly high protein consumption required for such large volume cultures. Membrane bioreactors, such as hollow fiber bioreactors (HFBRs), enable cell densities approximately 100-fold greater than traditional culture systems and therefore may enable a significant reduction in culture working volumes. As cultured cells, and larger molecules, are retained within a fraction of the system volume, via a semipermeable membrane it may be possible to reduce protein consumption by limiting supplementation to only this fraction. Typically, HFBRs are complex perfusion systems having total volumes incompatible with bench scale screening and optimization of stem cell-based cultures. In this article we describe the use of a simplified HFBR system to assess the feasibility of this technology to produce blood products from umbilical cord blood-derived CD34(+) hematopoietic stem progenitor cells (HSPCs). Unlike conventional HFBR systems used for protein manufacture, where cells are cultured in the extracapillary space, we have cultured cells in the intracapillary space, which is likely more compatible with the large-scale production of blood cell suspension cultures. Using this platform we direct HSPCs down the myeloid lineage, while targeting a 100-fold increase in cell density and the use of protein-free bulk medium. Our results demonstrate the potential of this system to deliver high cell densities, even in the absence of protein supplementation of the bulk medium.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 2 6%
France 1 3%
United States 1 3%
Unknown 32 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 19%
Student > Ph. D. Student 6 17%
Student > Bachelor 4 11%
Student > Master 4 11%
Other 2 6%
Other 4 11%
Unknown 9 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 28%
Medicine and Dentistry 4 11%
Biochemistry, Genetics and Molecular Biology 3 8%
Chemical Engineering 2 6%
Engineering 2 6%
Other 6 17%
Unknown 9 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2020.
All research outputs
#2,536,235
of 22,710,079 outputs
Outputs from Cell Transplantation
#148
of 1,754 outputs
Outputs of similar age
#17,197
of 165,343 outputs
Outputs of similar age from Cell Transplantation
#1
of 28 outputs
Altmetric has tracked 22,710,079 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,754 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 165,343 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.