Title |
Atlas of Gene Expression in the Developing Kidney at Microanatomic Resolution
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Published in |
Developmental Cell, November 2008
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DOI | 10.1016/j.devcel.2008.09.007 |
Pubmed ID | |
Authors |
Eric W Brunskill, Bruce J Aronow, Kylie Georgas, Bree Rumballe, M Todd Valerius, Jeremy Aronow, Vivek Kaimal, Anil G Jegga, Jing Yu, Sean Grimmond, Andrew P McMahon, Larry T Patterson, Melissa H Little, S Steven Potter |
Abstract |
Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development. |
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Geographical breakdown
Country | Count | As % |
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United States | 6 | 4% |
India | 2 | 1% |
United Kingdom | 1 | <1% |
Australia | 1 | <1% |
Unknown | 142 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 47 | 31% |
Researcher | 28 | 18% |
Professor > Associate Professor | 15 | 10% |
Professor | 13 | 9% |
Student > Doctoral Student | 7 | 5% |
Other | 20 | 13% |
Unknown | 22 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 62 | 41% |
Biochemistry, Genetics and Molecular Biology | 27 | 18% |
Medicine and Dentistry | 23 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Business, Management and Accounting | 2 | 1% |
Other | 11 | 7% |
Unknown | 24 | 16% |