| Title |
TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants
|
|---|---|
| Published in |
Plant Molecular Biology, May 2013
|
| DOI | 10.1007/s11103-013-0078-4 |
| Pubmed ID | |
| Authors |
Toni Wendt, Preben Bach Holm, Colby G. Starker, Michelle Christian, Daniel F. Voytas, Henrik Brinch-Pedersen, Inger Bæksted Holme |
| Abstract |
Transcription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of organisms. The primary advantage of TALENs over other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their ease of assembly, reliability of function, and their broad targeting range. Here we report the assembly of several TALENs for a specific genomic locus in barley. The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, single-strand annealing assay. The most efficient TALEN was then selected for barley transformation. Analysis of the resulting transformants showed that TALEN-induced double strand breaks led to the introduction of short deletions at the target site. Additional analysis revealed that each barley transformant contained a range of different mutations, indicating that mutations occurred independently in different cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Argentina | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 145 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 1% |
| Italy | 1 | <1% |
| Cuba | 1 | <1% |
| Germany | 1 | <1% |
| Paraguay | 1 | <1% |
| Brazil | 1 | <1% |
| Spain | 1 | <1% |
| Denmark | 1 | <1% |
| Unknown | 136 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 29% |
| Student > Ph. D. Student | 25 | 17% |
| Student > Master | 22 | 15% |
| Student > Bachelor | 12 | 8% |
| Professor | 9 | 6% |
| Other | 16 | 11% |
| Unknown | 19 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 86 | 59% |
| Biochemistry, Genetics and Molecular Biology | 29 | 20% |
| Computer Science | 2 | 1% |
| Engineering | 2 | 1% |
| Immunology and Microbiology | 1 | <1% |
| Other | 3 | 2% |
| Unknown | 22 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2023.
All research outputs
#4,688,171
of 23,477,147 outputs
Outputs from Plant Molecular Biology
#380
of 2,858 outputs
Outputs of similar age
#39,477
of 197,083 outputs
Outputs of similar age from Plant Molecular Biology
#2
of 15 outputs
Altmetric has tracked 23,477,147 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,858 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 197,083 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.